Print ISSN: 1818-5746

Online ISSN: 2313-4429

Volume 17, Issue 1

Volume 17, Issue 1, Winter and Spring 2018, Page 1-146

Clinical and diagnostic study of sheep Pneumonic pasteurellosis in Basrah, Iraq

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 1-5

The study included clinical investigation on pneumonia that caused by Mannheimia haemolytica (M. haemolytica) in sheep of Basrah Province, also isolation and identification was done and confirmed the diagnosis by PCR technology. The blood samples and nasal swabs were collected from 410 local sheep breeds of both sexes, and different ages. The results showed that from 410 sheep there were 25 healthy against clinical and cultural tests, which concerned as a control group. The rest 385 sheep were revealed clinical pneumonia. The most important pneumonic signs included coughing, fever, abnormal lung sounds, dyspnoea, depression, mucopurulent nasal discharge as well as loss of appetite and separated from the herd. The laboratory bacterial culture and biochemical tests for samples from 385 pneumonic sheep appeared M. haemolytica in 81 (21 %) cases, which characterised by moist, round, white or grey colony with β-type haemolysis on blood agar. On MacConkey agar showed pink–red pinpoint colonies. While when stained by gram stain appeared as pink, short rods or coccobacilli and bipolar in methylene blue stain. The biochemical reactions included negative indole, urease, and citrate whereas positive for oxidase and catalase tests. The PCR technique indicated that from 81 isolates there were 48 59, 2% cases had evidence by Rpt2 gen as M. haemolytica in local sheep of Basrah Province.

Recovering cultivation-based identification of Escherichia coli from poultry in Al-Diwaniyah, Iraq

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 6-8

Aiming at Escherichia coli-based identification, the current work was intended to recover this microorganism from chickens affected by avian pathogenic Escherichia coli (APEC). To perform this an investigation from July to November -2015, 200 chicken-gut-based specimens were collected from different locations in Al-Diwaniyah City, Iraq. The affected chickens first investigated for macroscopic lesions such as on the external surface of the lungs that belonged to the affected birds. Gut-related parts such as duodenum suffered characteristic features of inflammation-based signs. Then, samples were cultivated on media agars for recovering APEC, detecting of infection rate, identifying active antimicrobial agents for better treatment. The sick chickens suffered various health problems such as intestinal inflammation and air sacculitis. Using MacConkey, TBX agar, and API-20E, the results showed the presence of these pathogenic microorganisms in the infected parts of these chickens.

Comparative study between two media on in vitro maturation rate of local buffalo oocytes

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 9-16

Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production and application of related biotechniques. The present study has been undertaken to study the effect of TCM-199 and synthetic oviduct fluid (SOF) medium with supplements (10% Bovine serum albumen (BSA), 10 IU/ml follicle stimulating hormone FSH) with100 IU/mL penicillin, 100 mg/mL streptomycin and 100 IU/mL Nystatin on maturation of buffalo oocytes in vitro. Buffalo ovaries (n= 300) were collected from local abattoir of Basrah within two hours of slaughter and transported immediately to the laboratory under septic condition. Follicular oocytes (n= 215) were recovered by aspiration and slicing method. Only normal grades(A and B) cumulus-oocyte-complexes (COCs) were incubating under 5 % CO2 in air at 38.5 C for 24 hours in two media TCM-199 media (n= 105) or SOF media( n =110) oocytes adding same supplements which mention above. The percentage of oocytes maturation when using TCM-199 medium was (33.33%) was significantly higher (P<0.01) compared to those obtained with SOF medium 13.91(%).

Impact of Fascioliasis on circulating physiological biomarkers of stress and inflammation in cattle

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 17-22

This study was conducted for verifying the effect of liver worm sickness (Fascioliasis) on the general health of the animal, The hematocrits levels, (RBCs) count, Haemoglobin concentration ( Hb ) and packed cell volume (PCV) were also determined in the two groups of beef infected and non-infected with Fascioliasis the inflammatory parameter in liver :glutamic oxaloacetic transaminase (GOT) and glutamate pyruvate transaminase( GPT) were determined in the beef with moderate Fascioliasis and The results of this study were compared between infected animals and non-infected animals. A few have been taken oxidative stress: Superoxide Dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA) and catalase enzyme (CAT). In both groups, there were some significant contrasts in the levels of GSH, MDA, SOD, CAT and GOT, GPT. For CAT there was a significantly accelerated in its levels in the infected beef in comparison with the non-infection beef. While GSH and MDA there was a decrease in the level of GSH and an accelerated in the level of MDA in compared to non-infected beef respectively. SOD activity was decreased in blood serum of infected beef comparison with non-infected. The findings showed the beef infected with F. spp. were under oxidative stress. In inflammatory parameters were some significant differences in the levels of (GOT, GPT), its showed accelerated in levels with infected animals and normally in non-infected animals. This research reinforces that animals infected with trematodes, which may have effects on the overall health of animals, will be under oxidative stress and inflammation.

A comparison between Aloe vera and silver sulfadiazine on second-degree burns in local male rabbits: A Histological study

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 23-28

The present study carried out on fifteen local male rabbits six months in age, the animals divided for three groups five animals for each, after general anesthesia of all animals, the thoracic region was exposed for three seconds to 2 cm2 wide electrical heating rod to reach second-degree burn. The first group exposed to Vaseline only as a sham control group, second group exposed to silver sulfadiazine cream 0.2% and third group exposed to the Aloe vera (85%), all groups treated continuously once time for fifteen days, and specimens collected from affected area during 5,10 and 15 days after treatment for histopathological evaluations. The results of the study revealed semi like frequent healing process in all groups after five days, whereas at the ten days after treatment the second and third groups showed noticeable healing processes than control group, while at fifteen days from treatment the animals treated with Aloe vera showed approximately complete healing process characterized by presence of thick keratinized layer with profuse collagen in the dermis as well as new formation of hair follicle with noticeable hyperplasia of epidermal layers when compared with second and first groups.

Effect of probiotic on humoral immunity of broiler chickens vaccinated against Gumboro disease under experimental aflatoxicosis

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 29-34

The aim of this study was to investigate the effects of supplementing broiler diets with probiotic live yeast, Saccharomyces cerevisiae, (SC) to alleviate the deleterious effect of Aflatoxin B1 on the immunity of broiler chickens after Gumboro disease or Infectious Bursal Disease (IBD) vaccination. A total number of 120 unsexed Hubbard broiler chicks were used in this study. The chicks were randomly divided according to diet supplementation into four groups (30 chicks for each) from 1 day old to 6 weeks of age as follows, G1: Basal diet (control), G2: basal diet + SC 2g/kg, G3: basal diet + AFB1100µg/kg, G4: basal diet + AFB1100 µg/kg + SC 2g/kg. All groups were vaccinated with IBD vaccine at age of 14 and 28 days old. Blood samples were collected to test the immune response by ELISA test at 20 and 35 days old. The addition of SC to the broiler diet G2 revealed significant enhancement of the immune status. While the results of G3 showed that AFB1 at 100 ppb broiler diet caused significant (P<0.05) decrease in the value of antibody titers of IBD immunity at 2 examined periods of age, although G4 AFB1 plus 2g/kg of SC showed a Tangible role in minimizing effects of AFB1. It is concluded that AFB1 at 100ppb does have a negative effect on IBD vaccination while using SC had the ability to elevate the immune response after IBD vaccination with and without AFB1 contaminated diet.

Effect of addition of different levels of pomegranate peel powder to concentrate diet on productive performance of Awassi lambs

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 35-43

This study was conducted to investigate the effect of the addition of pomegranate peel powder to concentrate diet at a level of zero (T1), 1.5 (T2) and 3% (T3) on productive performance of Awassi lambs. The concentrate was offered to lambs at a rate of 2.5% of live body weight and ground wheat straw on the adilbitum basis. Results revealed that there was a significant (P<0.05) increase in straw dry matter, organic matter and nitrogen intakes by lambs fed the low level of pomegranate peel powder (T2), whereas, lower values were recorded by lambs fed the high level (T3). With the similar trend of change, total dry matter intake were 1056.03, 954.61 and 841.48 g/day, and 975.35, 896.24 and 793.92 g/day of total organic matter intake, and 8.49, 7.73 and 6.70 g/day of total nitrogen intake for treatments 2, 1 and 3 respectively. Although there was no significant effect in growth parameters, lambs fed T2 gained better final weight, total and daily gains, 34.20, 8.15 and 145.53 g/day respectively, however, lambs fed T3 recorded 31.52, 5.72 and 101.34 g/day for these parameters respectively. Lambs fed T1 and T2 achieved better values of feed conversion ratio as compared with lambs in T3. Lower digestion coefficients were recorded by lambs fed T2 as compared with lambs fed T1 and T3 with a slight difference in dry matter digestibility (58.39%) and organic matter digestibility (59.68%), and relatively high differences in crude protein digestibility (51.78%) and nitrogen-free extract digestibility (63.85%). Lambs fed T2 were prior in ether extract digestibility in comparison with lambs fed T3, where digestion coefficients were 59.92% and 55.09% respectively vs. 60.17% for control treatment. Crude fiber digestion coefficients were closed among the three treatments.

Clinical and immunological effects of experimental infection with Klebsiella pneumoniae in lambs in Iraq

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 44-48

The study was conducted on ten Awassi male sheep to study experimentally pathological changes of Klebsiella pneumoniae infection during (3 months). Experimental animals were divided randomly into equal groups(each group included 5 animals), induction of pneumonia in one of these group in sheep by intratracheal route with the infective dose (3 ml) of bacterial suspension in concentration (105 CFU/ml) & the second represent as control and gave a sterile D.W. in the same dose & route of infection) and clinically exam daily with weekly complete blood indices, humoral and cellular immunity were examined by passive hemagglutination test and phagocytic activity as well as histopathological examination of lungs from two scarified sheep in the first group. Nasal discharge appeared as serous then turned to mucopurulent, intermittent cough, mild fever, increase in pulse and respiratory rates (42.4±1.39 and 35.2±1.77 respectively with significant differences between the two groups. WBC count increased significantly in the infected group as compared with the control group. GRN% showed statistically a significant increase in the values reached to (8.88±0.86) during that period of infection compared with control and Mon% showed a statistically significant increase in values during that period in the infected group, and the Abs titration was the higher in the infected group significantly than control and the cellular response in the infected group revealed increment in phagocytic index of neutrophils than in the control group.

The effect of some additives on histological changes of broiler chicks

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 49-55

This study was conducted to investigate the effects of feed additives on weight and histological changes in the small intestine. A total of (350) broiler chicks one –day old (Ross 380) – unsexed were randomly divided into (7) equal dietary treatment groups, as follows: Treatment (T1): Basal diet without any addition (control group) ,Treatment (T2): Basal diet + Garlic 4cc/10L,Treatment(T3):Basal diet + Saccharomyces cervisiae 2.5gm/10L,Treatment (T4): Basal diet + Stpire 2.5cc/10 L, Treatment (T5): Basal diet +B. glucan 8gm/10 kg, Treatment (T6): Basal diet + Levamisole 1gm/kg, Treatment (T6): Basal diet + Levamisole 1gm/kg, Treatment (T7): Basal diet + Neomycin 0.5 gm/L. These additions were presented on the first day of the arrival for 5 days, and on the day of first vaccination for 5 days and at the end of the experiment for 5 days also. These chicks vaccinated with NDV on the day one of age by injection in the neck and re-vaccinated at day 10 of age with Lassota, Body weight was weekly determined the results as following: Chicks feed a diet with (T7) showed improvement in villus height and in the (T3, T4).

Comparative study on locally produced bivalent inactivated Newcastle disease vaccine in broiler chicks

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 56-63

The study included titration of the Newcastle disease virus strains before the preparation of the vaccines used in this experiment, by detection of EID50 for these strains. This study regarded the first one in the country in preparation double strain oily Newcastle disease virus vaccine. The immune responses for this vaccine were compared with those of single strain NDV vaccine. The same vaccination program was used for all groups included in this study in which live attenuated) Lasota strain) vaccine given at one day old as an installation by eye and nostril, and subcutaneous injection of oily vaccine at the neck region. A total of 120 commercial broiler chicks was divided into fourth groups, (1th) group vaccinated with live attenuated vaccine and bivalent(B1, Lasota )oily vaccine while the (2th) group vaccinated with live attenuated vaccine and (Lasota)oily vaccine and (3th) group vaccinated with live attenuated vaccine and (AG68 )oily vaccine, (4th) group C was left unvaccinated as control, the Immune response for all groups was measured at( 1) day old, (7), (14), (21),and ( 35) days old by hemagglutination test (HI) and Enzyme liked immunosorbent assay (ELISA). The results showed gradual recreation of immune response titer at 7,14 and 21 days post-vaccination intervals with non-significant differences (P> 0.05) among vaccinated groups at (7,14) days and significant difference between vaccinated groups and control group (P>0.05) at (21) days old.T he third group reflected better titer among vaccinated groups, and the result also shows increased antibodies titer at (35) day in comparison with antibodies titer at (14,21) days intervals. With statistical differences (P>0.01) among (third) group and second, and control group). However all groups gave higher titer than the control group (P>0.01) and the result of (HI) test agreement with (ELISA) test while the protection percentage was measured by challenge test ,it was found to be ( 100 % ) for group (3th) vaccinated with live attenuated vaccine and bivalent(B1, Lasota )oily vaccine, (92%) for the second groups (2th) (96%) for the first groups (1th) whereas control group (4th) gave (10%) protection. This study indicates the efficacy of prepared ND vaccines (B1, Lasota) as compared with monoclonal vaccines (B1, Lasota) and the high immunosuppressive capacity of the oily vaccine increased with virulence.

Anatomical study of fore limb extensor and flexor tendons below the carpal joint in Iraqi goats (Capra Hircus)

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 64-70

The present work includes the anatomical study of forelimb below the carpal joint in Iraqi goat. It was performed on (10) forelimbs of five Iraqi healthy goats, with no history of lameness and without local swelling or joint distension. Results showed the limbs of goat comprise of extensor and flexor tendons. Extensor tendon was located on the dorsal surface of the limb and no communication between the medial and lateral extensor tendons. The common extensor tendon in a goat was divided into two branches in the distal region of the metacarpal bone, one for each digit continues and inserted in the phalanx III (PIII). The medial and lateral extensor digital tendon was inserted on the proximal extremity of phalanx II (PII). While the flexor tendons located on the palmar surface of the limb upon the suspensory ligament. Superficial digital flexor tendon inserted on the palmar surface of (PII), while deep digital flexor tendon continues on the (PII) and inserted on the flexor surface of (PIII).

Anatomical and histological study of the thymus gland in native rabbits (Oryctolagus cuniculus)

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 71-74

The aim of this study is to add the available information of the anatomy and documenting the normal microscopic features of the thymus gland in rabbits, for this reason, ten rabbits from the local breed aged between (5-7) weeks breeding in animal house. After anesthetic the healthy animals intramuscularly in the thigh, the thoracic cavity opening by surgical scissor, to entering the thymus to study it anatomically and collected the specimen for histological study. The animals divided into two groups: the first group for the anatomical a study and the second group for histological study, the results appear that thymus of rabbit occupy most the thoracic inlet, pyramid in shape, white to yellowish in color, located rostral of the mediastinum area which covered the large vessel of the heart and aortic arch. The thymus is lobulated organ invested by capsule contain from loose connective tissue each the lobe consist of two different area, the peripheral area was deep stain called cortex and the inner pale area called the medulla, which contains different cells of lymphocyte and epithelial cells which noted arranged in concentric layers forming Hassall ś corpuscles.

Isolation and identification of Salmonella serotypes in poultry

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 75-80

Our study was designed to investigate the prevailing serotypes of Salmonella in chickens and the rate of isolation of each serotype. Between 27 September 2014 and 15 February 2015, a total of 200 samples were collected from cloacal swabs and cecal contents from different sources and ages at Al-Diwaniyah Province. The bacterial culture was carried out by using different culture media such as Salmonella-Shigella agar (SS), Xylose Lysine Deoxycholate Agar (XLD) and Chrome agar Salmonella (CAS), the serotype was determined by confirmative bio-chemical essays using the Vitek-2 system. The current study was able to identify three serotypes as following: S. enteritidis, S. Paratyphi B and S. typhimurium with isolation rate of 10%, 6.5%, and 4.5% respectively.

Escherichia coli strains as Major secondary bacterial pathogen isolated from an outbreak of swollen head syndrome in layers, in Al-Diwaniyah, Iraq

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 81-88

A cross-sectional study was conducted to identify the involvement of E. coli as a secondary pathogen in cases of Swollen Head infection and to detect drug susceptibility pattern of these isolates. This study was carried out on 20 chickens that were purchased from Al-Safaa Company from an outbreak of a sudden increase in mortality in layers farm and experienced clinical signs included difficult breath, coughing, rales, swollen of infraorbital and supraorbital sinuses, and conjunctivitis, as well as severe depression. The main gross lesions demonstrated among the infected chicken including gaseous exudate in the trachea, nasal passages, and sinuses. There was yellowish gaseous exudate on the air sacs, ovaries, and the peritoneum. The samples were inoculated on different bacteriological culture media, the isolates were identified by morphological, and biochemical tests, in which the result revealed that the major pathogens associated with the swollen head syndrome in layers were E.coli. The majority of isolates were resistant to ciprofloxacin (94.4%), Ampicillin (100%), Erythromycin (100%), azithromycin (100%), trimethoprim (88.9%), and levofloxacin (94.4%). Nonetheless, however majority of APEC isolates were susceptible to nitrofurantoin (72.3%). Moreover, all E.coli strains recovered in the current study showed multidrug-resistant to three or more different antibacterial classes. In conclusion, the present findings showed that MDR E. coli is prevalent SHS. The MDR E. coli is an alarming signal because these bacteria can transfer their MDR trait to potential human and animal pathogens. Therefore, the introduction of surveillance programs to monitor antimicrobial resistance strains is strongly recommended to protect human and animal health.

Detection of some heavy metals (Pb, Cd, Ni and Hg) in some animals meat of local markets

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 89-93

The present study involved detection for concentration of some heavy or toxic metals which have pathological effect on the human population if consumed contaminated meat with these metals (60) samples collected for goat meat (n=30) and camel meat (n=30) from butchers shops of markets districts (Al-Hamza, Al-Daghara and Afak) in Al-Qadisiyah province whose slaughter the animals from these districts only. The result showed a significant variance (p≤0.05) in concentrations of (Hg, Cd, and Ni) in goat meat were (0.0820, 0.1197 and 0.1099 mg/kg) respectively while Cd concentration not recorded significant variation studied districts. While the concentration of Hg and Pb recorded significant variance (p≤0.05) in camel meat in Al-Daghara district compare among study district were (0.1200 mg/kg and 0.3477mg/kg) respectively and we did not show dramatic a significant variance in Pb and Ni concentrations in the study regions. With regarded of animal factor we showed significant variance ( p≤0.05) in concentrations of Pb and Ni in goat meat compared with camel meat were ( 0.9975 mg/kg and 0.5960 mg/kg ) respectively while the concentration of Hg and Cd was not recorded significant variance between the meat of goat and camel.

Pathological effects of mercury chloride on reproductive system in white rats

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 94-99

The present study was undertaken to know the reproductive toxicity of mercury chloride in male and females of white rats. This study was used twelve (6 male and 6 females) white rats of approximately of the same body weight (200-220 g) divided equally in to 3 groups; the first group (T1) was received mercury chloride (1mg/kg B.W intraperitoneally once daily for 30 days). While the second group (T2) was received, mercury chloride (1.5mg/kg B.W intraperitoneally once daily for 30 days).Third group was received only0.2ml of Distilled water considered as control group. At the end of an experiment, the animals were sacrificed and small pieces of (2cm³) were taken from ovary, uterus and testis of all animals to histopathology. Histopathological sections of these organs of (T2) group was showed severe pathological changes characterized by vaculation in epithelial cells of uterus, cystic dilatation of uterine glands with degeneration of epithelial lining of its ,hemorrhage and decrease in number of growing follicles in ovary also there were sever pathological changes in the testes. While (T1) group was showed less pathological changes characterized by hyperplasia of epithelial lining with few and small uterine gland in uterus, presence of large secondary follicles in ovary and there were less pathological changes in testes.

Castration of Iraqi local bucks by unilateral spermatic cord torsion compared with castration by Burdizzo

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 100-110

The study aimed to investigate the efficacy of using unilateral spermatic cord torsion as one of the easy castration techniques in Iraqi local black goats, also to study the effect of unilateral spermatic cord torsion on the contralateral testis. Fifteen local male black goats (adult bucks 13 - 15 months weighing (31.9 ± 3.25 Kg) were divided into three equal groups. The first group was left without treatment as a control. The second group was submitted to a unilateral (right) spermatic cord torsion. The third group was submitted to bilateral castration by Burdizzo. Weighing of animals and semen were collected from bucks before castration, and after two months of castration, for seminal analysis. In addition, blood samples before castration, after two months of castration and two weeks after orchiectomy were taken for determination of the serum testosterone hormone level. After two months of castration, both testes were orchied surgically in all animals for studying the weight, dimensions and histopathological changes of testes. For that, it can be used as a technique for castration since it was easy and with fewer complications. As well as the unilateral spermatic cord, torsion causes a damage to the contralateral testis.

Sheep coenurosis in Thi-Qar Province, Iraq

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 111-114

Coenurus cerebralis, the metacestode or larval form of the dog tapeworm Taenia multiceps, causes coenurosis, otherwise known as gid or stagger. C. cerebralis causes a serious problem in sheep production. Prevalence of Coenurus cerebralis in sheep herds in different times (2004-2017) and locations and causes in characteristic clinical signs and an important percent of deaths. The diseased sheep were used for clinical examination and anatomy in five herds (1610 heads) of sheep at different times and from different areas in Thi-Qar province south of Iraq. All sheep were examined clinically in the herds during the study.T otal infestations by T. multiceps percentage where 13.29% in this study. The brain examination showed the presence of cysts of T. multiceps larvae in three places on the brain in the right and left hemispheres and at the bottom of the brain. The cysts were identified as being partially outside of the brain tissue and were buried in the brain tissue and were strongly attached and after separation leave a gap of size and a large number of the visible scolices (120-170 Scolices) in the form of white pieces, 1-2 mm long, similar to the lipid tissue. Microscopic examination of the scolices showed the presence of scolex of the Taenia multiceps and the movement of the larvae. The number of larval stage bags in the infected animal reached 1-8 cases. The infestations included lambs, which can be infected with the age of 4 months and adult sheep of both sexes. Our study showed a prevalence of coenurosis among sheep in Thi-Qar province and confirmed its diagnosis by used clinical signs, case history, brain anatomy and microscopic examination of C. cerebral cysts.

Measurement of some heavy metals in tissues infect by intestinal worms of two kind of fishes in Al-Qadisiyah Province

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 115-124

The present study has been conducted in four parts of AL-Qadisiyah (Al-Diwaniyah center, Shamiya, Hamza and Afak). A sample of 945 fish of Barbus grypus and Liza abu has been examined. It was found that 205 of the Barbus grypus fish and 344 of the Liza abu were infected with parasitic intestinal worms. Some parasitic intestinal worms have been detected such as the Neoechinorynchus iraqensis in both fish types. The female paulisentus, Bothriocephalus acheilognathi were found in both Barbus grypus, Liza abu and the larva of Contracaecum spp was seen in the Liza abu and Barbus grypus type. Four heavy metals were measured. They included Cadmium, Lead, Zinc, Nickel, by means of an atomic absorption spectrophotometer issues from the a fore mentioned types of fish were measured for these heavy metals these tissues were from the liver, muscles, gills, from four districts of Al-Diwaniyah throughout the year, Cadmium registered high concentrations in autumn (12.67) Mg/ gm in the liver of the Barbus grypus. Lower concentrations of Cadmium were registered in winter (1.30) Mg/ gm in the liver of Liza abu. The Barbus grypus livers had high concentrations of lead in autumn (21.03) Mg/ gm in Hamza rivers, whereas gills of the Liza abu registered lower concentrations (6.43) Mg/gm in spring. The livers of the Barbus grypus registered high concentrations of zine in autumn which where (32.27)Mg/ gm in Hamza river while Shamiya river they dropped to 21.17 Mg/ gm in spring, It was observed that Nickel had high concentrations in the livers, muscles, gills, of both fish types, The highest of which was in autumn, the Hamza river had the highest concentrations in autumn which were (29.17,28.63,27.67) Mg/g in Barbus grypus , in spring the nickel concentrations were low in the gills of the Liza abu11.10 Mg/ gm in Shamiya river.

Seasonal histological effects on the Leydig and Sertoli cells in the testis of indigenous dogs (Canis Familiaris)

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 125-131

This study was performed on twenty adult male dogs aged 2-3 years old. Samples of testis tissue were taken during the four seasons in 2016 in Iraq. Specimens were fixed in 10% formalin. Routine histological techniques were carried out for light microscopy. H&E and PAS stains were used. During active seasons (spring and autumn) the study revealed a significant increase in the number and diameter of Leydig and Sertoli cells compared to other inactive seasons (summer and winter). Cytological indications of mitotic events of Leydig cells were clearly observed, whereas, Sertoli cells never showed any mitotic divisions. The basement membrane of seminiferous tubules appears wavy with impacts of irregular gates of cellular penetrations. It was characterized by a dynamic, flexible structure, and can respond easily to the cellular mechanical pressure. The new generation of Leydig cells was derived either from the differentiation of interstitial fibroblast-like cells or from the mitosis of Leydig cells themselves; whereas the new generation of Sertoli cells was provided only by the differentiation of interstitial fibroblast-like cells. The study suggested that, after stimulation, the compensatory fibroblast-like cells can penetrate the basement membrane and then differentiate to Sertoli cells, as the similarity between the two cells were very large. It was concluded firstly that the proliferation of Sertoli cells within the seminiferous tubules was done only by the penetration and differentiation of the interstitial fibroblast-like cells and not by mitosis of Sertoli cells themselves.

Clinical, microbial, histopathological and molecular investigation of interstitial pneumonia in camels in Iraq

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 132-137

In this study, twenty-four camels in abattoirs of Al-Najaf and Al-Qadisiyah provinces were suspected to have interstitial bronchopneumonia. Clinical signs revealed protracted neck, misery, inappetence, heart rate was elevated (44±0.29bpm) and breathing was irregular and rapid (23±0.27bpm) and fever (39.2±0.1). Moist crackles heard at auscultation. Transtracheal wash (TTW) were sampled for cytology and bacteriology to make the diagnosis; white blood cells count (WBC) was (1420±5.95cells/µl) with neutrophilia (48%), total protein (TP) records (355±4.29 mg/dl). Bacterial culture of the TTW revealed pure colonies on blood agar; which were recognized by the VITEK 2 compact device and confirmed using the conventional polymerase chain reaction (PCR) as Klebsiella pneumonia ssp pneumonia. Postmortem specimen gave three kinds of colonies: the same one in TTW, which was the causative pathogen, two others; Staphylococcus lentus and St. vitulinus; diagnosed biochemically by VITEK 2 compact. Histopathological dissections on postmortem samples found in the lungs of camels ranged only in 4-8 years old, discovered the presence of interstitial bronchopneumonia.

Effect of usage Iraqi camel colostrum extract on some blood parameters in albino rats

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 138-141

This research was conducted in the Animal House of the Faculty of Veterinary Medicine / University of Qadisiyah for the period from 1/10/2016 to 1/11/2016. The aim of this research is to find out the effect of using Camel Colostrum extract on some blood parameters in albino rats. Use of the colostrum extract from camels after delivery in the first three postpartum periods (1 hour), postpartum (6 hours) and postpartum (12 hours). In this study, 30 rats of males and females, ranging from 150-200 g, were randomly divided into five groups each group contains 6 rats, All groups were infected with E. coli except (C) group. After that, 3 animals were killed from each group after five days of infection and treated as follows: Control group(C) The standard and distilled water were given only,( zero) left untreated, (group 1) given 0.2 ml of colostrum extract was removed after 1 hour of delivery (group 6) given 0.2 mL of colostrum extracted After 6 hours of delivery, (group 12), which was given 0.2 ml of colostrum extract taken 12 hours after birth, The results of the experiment showed a significant effect (p<0.05) for the treatment with the presence of significant differences between the groups and the results showed that the superiority of the group (1) on the other groups, followed by the group(6) after 30 days of the experiment in improving the parameters of the doll due to the high proportion of antibodies in this groups.

A new approach for estrus induction in Arabian mares suffering from ovarian inactivity

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 17, Issue 1, Pages 142-146

Twenty-six Arabian mares affected with ovarian inactivity, as monitored by the clinical examination and ultrasonographical scanning, were selected during December and January. These animals were subjected to a new protocol of sequential hormonal injections as a trial for estrus induction, progesterone (300 mg/day) was injected on days 1,4,7,10 and 14, Estradiol benzoate (15mg/day) on days 6 and 9, and GnRH (0.01mg/day) on days 18, 20, and 22 of the treatment regimen. Anestrus induction response of 80.77% (21/26) was observed following hormonal treatment. The onset time of post-treatment was short (66.48 hours) and more synchronized. The responded group showed significant (P<0.05) increase in follicular size from the day -3 (3.26 cm) until reaching its maximum size at the pre-ovulatory day (4.48cm). This was accompanied by continuous significant (P<0.05)decrease in the serum concentrations of progesterone and increase in the levels of serum estradiol-17β from the day -3 (0.42 ng/ml and 56.48 pg/ml, respectively). The minimum level of progesterone (0.28 ng/ml) was detected at day 0 of ovulation, whereas the maximum level of estradiol-17β was observed at the preovulatory day (89.22 pg/ml). However, 28.57% (6/21) of the responded mares did not develop functional corpus luteum so confirmed by functional progesterone estimation. Reasons for failure of 19.23% mares (5/26) to respond to hormonal treatment could be due to the small size of emerging follicles at the end of treatment (1.05 vs 1.82 cm ) as well as the older ages of these animals (15.36 vs 8.87 years old) compared to the responded group.