Print ISSN: 1818-5746

Online ISSN: 2313-4429

Volume 12, Issue 1

Volume 12, Issue 1, Winter and Spring 2013, Page 1-186

Study the effect of using baker's yeast(Saccharomyces cerevisiae) on serum glucose level and some hematological traits in kids of local goats

Kh. Gh. Alrikabi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 1-5

The experiment was conducted in one of the commercial animal farm located in QalatSiqar town which belongs to Thi-Qar governorate during aperiod from 2-3-2011 to 29-4-2011.This study was aimed to knowledge the effect of adding baker's yeast Saccharomyces cerevisiae (SC) as 3gm yeast/kg feed on the level of serum glucose and some hematological traits(RBCs count , WBCs count, PCV, Hb, ESR) in kids of local goats, twelve male kids were used in this study which are divided randomly into two groups (six kids for each group) , the results can summarized as follows:
1- Increase the level of serum glucose (non significant).
2- Numerical increase in RBCs count.
3- There were no difference among other hematological traits studied.

Incidence of Salmonella and Brucella of cattle in Baghdad

S. S. Salman; D.S. Jassim

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 6-10

Serological investigation was conducted on (100) serum samples. (50) samples were collected from aborted cows and (50) samples from non-aborted cows in some areas in Baghdad to detect the infection of Brucellosis and Salmonellosis by using Rose Bengal plate test and tube agglutination test for brucellosis and the Widale plate test for salmonellosis.This study was conducted on two axons, first axon to detect the incidence of infection with brucellosis and salmonellosis in cattle, while the second axon to detect the cases of mixed infection with salmonellosis and brucellosis.Rose bengal test conceders one of the most important screening test in detecting brucellosis in both aborted and non-aborted cows. The positive results in the RBPT were (32) with percentage (%64) in aborted cows and (22) with percentage (%44) in non-aborted cows, while the tube agglutination test gave (22) positive results with percentage(%44) in aborted cows and (5) with percentage of (%20) in non-aborted cows. The widale test gave good results in the diagnosis of salmonellosis in cattle, the incidence in aborted cows was(%28) while in non-aborted cows was (%16).It was noticed that the percentage of mixed infection between brucellosis and salmonellosis was (%16) in aborted cows and (%12) in non-aborted cows and this is due to the presence of common and non-specific antigens between the two bacteria.

Surgical correction of some digestive system affection in ruminant

A. H. Allawi; O. A.Sediq; A. M.Sheet; O. Abd almageed

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 11-20

Diagnosis and treatment of digestive system affection in ruminant that brought to surgical department of veterinary teaching hospital of Mosul University from 1/7/2011 to 1/2/2012 were made.Clinical examination, diagnosis and treatment for (53) affection were made. The affection were traumatic reticule peritonitis (17) cases in ratio (32.075%), indigestion due to non-penetrated foreign body (12) cases in ratio (22.64%), ruminal fistula (6) cases in ratio (11.32%), self-suckling (4) cases in ratio (7.54%), check muscle paralysis (3) cases in ratio (5.66%), atresia ani , atresia ani etrecti and rectal prolapse (2) cases in ratio (3.77%) for each on, finally atresia recti,left side abomasum displacement, omasal impaction, chronic ruminal tympany, , lacerated cheek wound (1) cases in ratio (1.88%) for each one.14 animals were treated are inspected after surgical operation to study the efficiency of treatment were used. (85.714%) of these cases were responded to surgical correction.We reach concluded that digestive affection is sperated in large ruminant specially the females.

The Pathological effect of cestoda Raillietina cesticillus in small intestine of local domestic pigeons Coulumba livia domesticus in Basrah Province

Z. A.Hassan

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 21-26

This study included the collection of small intestine of local domestic pigeons infected by cestoda Raillietina cesticillus which isolated and diagnosted by Al-Idreesy (2010). Gross and histopathological changes was studied. The gross changes included enlargement of the duodenum. The microscopic finding of duodenal sections revealed presence of sections of mature cestode segment of the parasite attached to the mucosa of intestine, as well as inflammatory reaction of the epithelial layer including infiltration of inflammatory cells, there is clear degenerative changes of epithelial cells lining of the mucosal layer of the infected intestine.

The use of the hen،s Egg test – chorioallantoic membrane (HET- CAM)test as a novel in vivo model to study the effects of dexamethasone on a differential white blood cell counts

M. Kh. Shindala; M. B. Al-Mashhadany

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 27-31

The aim of the present study was to investigate the possibility to use the hen،s Egg test – chorioallantoic membrane (HET- CAM)test as a novel in vivo model to study the effects of dexamethasone on a differential white blood cell counts.The result revealed that, the blood smeared of chick embryos in age 13- 15 days of incubation had to the all types of mature leukocytes . Because of the short period of chick embryos were treated with dexamethasone (90 minutes ) only Showed did not significantly changes in leukocyte counts (lymphocyte, Heterophil, Monocyte, Esinophil and Basophil) in treated groups with defferint doses of dexamethasone ( 2 , 4 , 8 , 16 , 32 , 64 , 128 mcg gram of total egg weight, topically). We conclude for our study that, the (HET- CAM) test can be used as in vivo model to study the effects of dexamethasone on deferential leukocyte counts ,because of the blood smear have all types of leukocyte, also this study manifested this model have low cost accompanied with other laboratory animals.

Effect of enterocin produced by Enterococcus faecalis on bacteria that cause diarrhea in children and young sheep

H. S. Awayid; Kh.M.Khamas

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 32-44

A total of 300 samples (100 samples from feases healthy adult persons, adult persons suffer from diarrhea and100 feases healthy child and from feases children suffer from diarrhea and 100 samples from feases young sheep suffer from diarrhea ) , taken from various hospitals in the Baghdad city during the period from January to April 2012. All isolates were subjected to the cultural, microscopical, biochemical examinations by Vitek 2 for identification up to the species. The results showed that 40 isolates belonged to Enterococcus faecalis of which, 25 isolates from diarrhea cause and 15 isolates from normal flora , 31 isolates of which 18 isolates belonged to E.coli and 5 isolates belonged to E.coliO157:H7 and 8 isolates belonged to Salmonella spp. All isolates were subjected to cultural ,microscopical, biochemical examinations and used( API 20 –E) strip. and sensitivity of 31 isolates was tested against (11-9) Antibiotics. Results revealed that isolates showed multi resistance to antibiotics, All isolates of E.coli and E.coliO157:H7 ,Salmonella spp.resistant to some antibiotics for negative Bacteria and belonging to the group Enterobacteriaceae. And Detection of the ability of E. faecalis local isolates to produce enterocin by testing the inhibitory activity agar and broth in two media MRS,BHI and two method against bacteria cause diarrhea. And The results showed a variety of the local isolates in their inhibitory effect against bacteria cause diarrhea in children and young sheep by inhibitor zone between (12-20) mm and was MRS liquid and solid the best media from the brain heart infusion .

Inovo injection of water extracted propolis on hatchability and some physiological characteristics of broiler chickens

M. A.FAHAD; W. H. A. AL-Shemmmari

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 45-51

The research was conducted to investigate the effect of water extracted propolis on hatchability,body weights of hatched chicks as well as some hematological traits which were represented by packed cell volume,Hemoglobin concentration ,Heterophil lymphocyte ratio,in addition to evaluation of glucose, cholesterol and plasma protein .A total of 300 embryonated eggs,10th day of incubation were used in the experiment , those eggs were randomly allocated in to 5 treatments , each treatment included 2 replicates , 30 eggs of each replicate . Eggs were injected by water extracted propolis in to 3 treatments meanwhile ,other 2 treatments were left as control,treatments were arranged as follow:
T1: Negative control treatment .
T2 :Posative control treatment(0.1 ml of distilled water was injected in each egg).
T3: Eggs in this treatment were injected with 50 mg /ml of water extracted propolis (0.1 ml/egg) .
T4: Eggs in this treatment were injected with 100 mg / ml water extracted propolis (0.1 ml/egg).
T5: Eggs in this treatment were injected with,150 mg / ml water extracted propolis . (0.1 ml/egg) .
Results showed no significant effect of water extracted propolis on hatchability and percentages of deformed chicks , mean while, the extracted propolis led to a highly significant effect (p > 0.01) on body weights of hatched chicks which were 44.35, 44.60 and 45.75 gm / chick in the third ,forth and fifth treatment respectively. The results indicated significant effect of the extract on some hematological values such as packed cell volume , hemoglobin concentration,whereas, the Heterophil /Lymphocyte ratio was significantly improved(p>0.01)which were 0.29,0.28 and 0.26 in the third ,forth and fifth treatment respectively . No significant effect of the extracted propolis was noticed on the plasma glucose but the plasma cholesterol was significantly reduced to 166.76 , 164.14 and 158.80 mg /100 ml in the third ,forth and fifth treatment respectively, plasma protein was elevated significantly (p>0.01) due to the injection of the extract as well ,it was 3.19, 3.93 and 5.06 gm/100ml in the third ,forth and fifth treatment respectively .It was concluded that water extracted propolis had an important effect in increasing body weights of chicks post hatching as well as improving H/L ,reducing plasma cholesterol and elevation of plasma protein .
Key words : Proplis. In ovo injection.

Evaluation of Toxoplasmosis in feed lot lambs in Nineveh Provence

H. S Abdullah; I. I. Aldabagh; B. M. Jasim

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 52-57

The aim of this study was to evaluate the occurrence of toxoplasmosis in feed lot lambs in Nineveh Provence .the results indicates.A wide difference in the incidence of toxoplasmosis in the different areas of Nineveh and most infections was concentrate in areas near Tigris river and its low in the mostly dried areas . The infection percentage in the different areas of Nineveh was ranged from zero to 26.7 % and the total percentage was 7.3 % . The results indicate the importance of studying the epidemiological factors affecting the transmission of Toxoplasma as well as the effect of seasonal transportation of lamb in Nineveh and the availability of the source of Toxoplasmosis ( cat ) in different area of Nineveh Provence to get a better knowledge on the epidemiology of toxoplasmosis in lambs which helping in eradicate this disease .

The effect of cryptosporidium experimental infection on the immune response of broiler vaccinated with lasota

E. Y. Hussain; A. A. Shamoon

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 58-63

Two hundred forty (Ross-308) broiler chicks,Where equally randomally divided to six treatment groups;Each group composed of fourty broiler chicks. The first group kept as control,The second group inoculated Oocyst of Cryptosporidium at seven days,The third group inoculated Oocyst of Cryptosporidium at seven days and vaccinated with live lasota strian in ten days,the fourth group inoculated Oocyst of Cryptosporidium at seven days and vaccinated with live lasota strian in fourty days,The fifth & The sixth group vaccinated with live lasot strian in ten, fourtein days respectively. The parameters used in this study included: body weight,spleen,&bursa fabricious weight,mortalty rate,clinical signs,postmortem findings,histopathological examination and Humoral immuneresponses.The aim of this study is to know the interaction between expirmental Cryptosporidiosis with laSota strain in broiler chickens.Also the blood sample collected during the peroid (10,17,21,30,35)days to estimate the immune response by: The heamagglutenation in hibition and Enzyme-linked immunosorbent Assay (ELISA) test for the Newcastle disease of LasOta strian, The result of serological test have revealed the heamagglutenation inhibition test in the age 10 day it showed no siginificant, but differences at seventy day showed siginificant decrease in the fourth group in compare with the control group (4.25±0.479),in the (21,30,35)day it showed the increase siginificant in the fifth group(7.25±0.25,8.5±0.578,5.75±0.23).The revealed that the immune response by used indirectELISA if showed the decrease in the titer of the maternal antibody in the second group at 10 day, but showed the highest titers of antibody in the fifth group in the (17,21,30,35) days,Birds in all group showed no clinical signs,no mortality,no gross lesions,but the histo patological changes in bursa fabricious and intestine showed mor intensive in intestine 2nd,3rd ,4th groups,desequmination of intestine mucosa,cellular infiltration of inflamtory cell ,with present stages of parasite while of histo pathological lesions in bursa fabricious reveled hyperplasia,vacular degenration, presens of goblet cell in follicular&presense number of Cryptosporidium baileyi in the bursa mucosa. Conclusion:Infection by Cryptosporidium baileyi in broiler anegative influances on body weight and immunity against ND ,WUTH histopathology chenges in bursa of fabrious and intestine.

Histological study of the effect of Marek's vaccine on Newcastle and Infectious bronchitis vaccines on layers

H.M.Jaro; T.S.Qubih

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 64-71

This study was conducted to evaluate the histopathological effect of Marek's vaccine on chicken vaccinated by Newcastle disease and infectious bronchitis vaccines . 240 chicken were used divided in eight groups , and vaccine by different route at different ages . samples of spleen, Fabrius busra trachea and lung were taken at 7 , 14 , 21 , 28 , & 35 days for histopathology.The result indicated that there was variable change in groups , vaccinated with Newcastle disease and infectious bronchitis vaccines comparable to the negative & positive groups.

The incidence of Trichomonas in the dove (Streptopelia decaocto) and pigeon(Columba livia ) at Al-Diwaniya city and its effects on blood picture

A.A.A. Abed

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 72-77

This study was conducted to determine the incidence of Trichomoniasis in tow different types of birds at Diwaniya city , the effect of age on the incidence and the effects of parasite on some blood parameters traditional techniques for the detection of parasites in the birds were carried out by using a wet amount method for direct diagnosis , the research have been studied the spread of the parasite in tow types of birds as followed pigeons Columba livia , doves Streptopelia decaocto the results showed that from 100 pigeons, 29 birds were positive and 13 positive birds from 100 doves , the total percentage of positive was 29%, 13%,respectively .Effect of age on the rates of infection were clear as shown in this study, the percentage at pigeon and dove squabs were 62% and 30% respectively, while the percentage of infection of adult pigeons and doves were15.49% and 8.75% respectively with significance differences , t te results showed that there is some effect of Trichomonas on the blood Picture, by decreasing the concentration of Hb and PCV ,with increased of WBC significantly with no clear influence on RBCs.

Repeat breeder in Iraqi cows: Clinical and therapeutically study

Mohammad; A. H

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 78-82

The present study was conducted on 35 Iraqi cows suffered from repeat breeding , AL-yousfia in Baghdad province from Nov. 2009 to Nov. 2011, this ages ranged from 4-6 years.
The animals were divided randomly into three groups according to regimes of their treatment, the first group (13 cows) were treated by lugol’s iodine (50 ml ) intrauterine, the second group(11 cows) were treated by 2 gm of oxytetracycline 10% (20 ml Oxytetracycline + 20 ml distal water) intra-uterine and the third group (11 cows) were treated by 2 tube of Metricure (cefapirin , Arshark vet. products ).Results revealed that the number of cows responded were 84.6%, 81.8 % and 72.7 % in the 1ts , 2nd and 3rd group respectively. The mean of services per conception 3.62 ± 0.65 , 3.81 ± 0.83 and 3.96 ± 0.56 in the1ts , 2nd and 3rd group respectively and not recorded significant (p < 0.01) between groups but the days open were significant p <0.01for superiority in 1ts group comparable with 2nd and 3rd group.
The rotational of fetal membranes are recorded 25 % (7/28).In conclusion that the using of lugol’s iodine or broad spectrum antibiotics ( oxytetracycline or cefapirin) intrauterine therapy were very effective in the treatment of repeat breeder in Iraqi cows.

Partial Hepatectomy Achievement by New Device in Rabbits Model

W. A. Alheani; E. R. Al-Kennany

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 1-14

This study was to evaluate the efficiency of new device (heat gun) for liver surgery. This instrument is firstly introduce to the surgery to control bleeding during tissue dissection.Eighteen rabbits aged between 3-4 months weighted between 1.5- 2 kg from both sex were used in this experiment. The rabbits were divided into three groups. First group (A) underwent partial hepatectomy by heat gun to posterior left lobe of liver and second group (B) underwent partial hepatectomy by using suturing of severed posterior left lobe of liver served as positive control . The third group (C) underwent laprotomy only and served as negative control.Postoperative examination of physiological parameters was measured for 3 postoperative days, The animal were scarified after 7, 15, 21 days after operation for gross and histopathological examination.The results revealed that efficiency of heat gun for partial hepatectomy and it was a fast method and prevented bleeding from the incision site and it is decreased time that needed for suturing hepatic incision in liver surgery.The physiological parameters in all the experimental rabbits were within normal range during the three days following the operations, and they gained their normal body activities and food intake.In gross pathological examination there is no serious complication that recorded except adhesion with omentum that is considered advantage for sealing incision and preventing adhesion with other neighboring viscera and prevent possible bleeding from hepatic incision. In histopathology the rabbits in group (A) show faster regeneration than in group (B).In conclusion, the heat gun is effective device for partial hepatectomy rabbits models and It is the first use for this purpose in this animals.

Study of some serum biochemical changes of experimental diabetes mellitus in Donkey

A. H. Fadhil; R. K. Muhsen; A. O. Ismael

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 15-23

This study was conducted to induce experimental diabetes in donkeys and assess some serum biochemical changes in diabetes mellitus.Fifteen donkeys were used in this experiment and divided into two groups:
A. Treatment group which include (11) donkeys . Diabetic group. denoted by Dm.
B. Control group which includes (4) donkeys denoted by Cm.
Animals were housed in field .The animals were left tow weeks for adaptation. Diabetes mellitus was induced by i.v injection of alloxan monohydrate at dose rate 100 mg/kg dissolved in 25 ml of normal saline, while the control groups were injected with 25 ml of normal saline. Blood was collected before injection of alloxan monohydrate and after that (10,20.30,40)days to check fasting and random serum glucose.The laboratory tests including serum glucose, serum urea, serum creatinine, serum cholesterol, serum lactate dehydrogenase , We found out the following result according to the periods of the experiments: Serum glucose level was elevated starting from the 1st 10 days period after induction of diabetes mellitus. It reached its peak levels on day(20,30,40). Serum urea, serum creatinine, serum cholesterol, serum lactate dehydrogenase were elevated in diabetic donkeys,in control groups, but there were no significant difference.

Detection of bovine corona virus in some governorate of Iraq

Kh. A. Mansour; S. A. Hasso; M. N. Al Rodhan

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 24-28

In the present study we used Reverse transcription polymer chain reaction (RT-PCR) assay for detecting of bovine corona virus ,We evaluated presence of bovine corona virus ( BCoV) in diarrheic fecal samples in age (1 – 30)day . (152) faecal samples from diarrheic calves were collected from four Iraqi governorates (Al-Qadisyia, Babylon ,Wassit and Najaf) .10 (6.57%) out of 152 were positive to bovine corona virus . This study is the first detection of bovine corona antigen in Iraq .The results suggest that RT-PCR is more sensitive than other method to detect BCoV, especially in subclinical cases and Because these animals shed a low amount of virus in faeces and more animal show clinically normal we need to apply sensitive techniques, such as RT-PCR .

Histopathological Studyof Trypanosomiasis in Camels of Al-Diwaniyah Province

A. H.A. Al-shabbani; Kh. A. Dawood; Gh. A.Jassem

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 29-35

The present study includes knowledge the histopathological changed of trypanosomiasis that is caused by Trypanosoma evansi in camels. Sample including: kidney, liver and spleen. The histological manifestation of the liver in the acute stage of trypanosomiasis shows coagulative necrosis, hemosiderin infiltration and billrubin.In the chronic stage shows infiltration of fibrous tissue and connective tissue between the lobules of the liver and decrease number of hepatic cells with hemorrhage, red blood cells among the hepatic cells.Lesions of spleen in the acute stage reveals hemorrhage and active lymphoid follicles with clumping of trypanosoma in spleen surrounded by inflammatory cells, in chronic stage fibrosis between the cells with increase thickness of trabeculae.In chronic stage of trypanosomiasis in kidney show glumerulonepheritic due to precipitation of immune complexes as homogenous and calcified glumerulus due to precipitation calcium with desquamation of epithelial cells which are lined the urinary tubules and full the lumen of tubules, glomerulus suffer from shrink of the tuft, increase thickness of Bowman’s capsule due to infiltration of fibrous tissue.

Dxtract of determination the therapeutiction of Datura metel leaves extract for some urinary system bacteria in rabbits (in vitro and in vivo )

H. K.Al-Mosoy

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 36-43

This study was designed to evaluate the effects the watery and alcoholic extracts from leaves of Datura metel in vitro to ten of pathogenic bacteria and study the watery extract toward Candida on urinary system in rabbits. The results showed the watery and alcoholic extracts from leaves of Datura metel have antibacterial activity against both Gram positive (Staphylococcus. aureus – Streptococcus .agylactiae ) And Gram negative bacteria (Klebsilla. pneumonia –Proteus. vulgaricus – E.coli – Pseudomonas – Vibrio – Salmonella – Enterobacter ) and Candida. albicans however the watery extract of Datura metel were more potent than alcoholic extract against pathogenic bacteria (80%) . According to results , Candida pathogen that more sensitive to ward Datura metel watery extract was choosing to injected intraperitonialy as experimental infection in laboratory animals (vivo) which cause morphological and histopathological degenerative lesion of kidney cortex and medulla tissue in addition to change of renal profile test that include blood urea nitrogen, creatinine, creatinine kinase, uric acid, in addition to Potassium. but after watery extract of Datura metel injected in these laboratory animals cause significant improvement (p≤0.01) in the value of blood urea nitrogen, creatinine, creatinine kinase and uric acid. Potassium concentration, histopathological studies confirm these results which include regeneration of degenerative lesion for medulla and kidney cortex with convoluted tubules tissue.

Isolation and characterization of streptococcus bovis from rumencontent of awassi sheep in iraq

A. J. A. Al emery; A. A. Yousif; H. A. Al-Hilaly

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 44-51

This is the first study in Iraq aimed to isolate and characterize Streptococcus bovis from rumen of Awassi sheep .Ten sheep with different ages fed on grain base diet for three days were used to collect 20 ruminal fluid samples twice at fourth and fifth days by rumenocentesis method , samples cultured on selective media (Modified membrane-bovis agar(M-BA) ,broth of basal medium and modified blood brain heart infusion) ,the isolates were identified according to their morphological, physiological ,biochemical tests and serological by Lancefield group.Cultural characteristic on the selective medium M-BA showed two types of streptococci :first type (23) comprised the majority of isolates ,this type characterized by high acid producing streptococci formed mucoid ,creamy ,orange –centered colonies and second type (4) characterize by low acid producing formed small white colonies .Morphologically the isolates were identified as gram positive ,the cells were oval or spherical , singles, pairs & short chains of 4 to 8 cells. The organisms were found to full under the Lancefield group D.All isolated bacteria grew on broth of basal medium post incubation at 45C˚ ,but not grew at 10C˚ and 50C˚. Adding of 2% NaCl permit the growth, while in 6.5% NaCl didn’t grow. And did not grew on adding 0.04 % Potassium tellurite .All isolates produced lactic acid but ammonia production from arginine was negative, no hemolysis on blood agar . All isolates ferment starch, lactose, fructose, glucose, raffinose and cellobiose.The results of culturing and Physiological and biochemical tests showed that 27 isolates has the characterictices of Streptococcus bovis in 3 types (S1,S2 &S3) ,first type of colony divided into two strain (S115)(S28)according to difference In arabinose. And (S3) called on the second type white pigmented isolates which did not ferment inulin

Morphological study of the Larynx of the indigenous adult Male Pigeon (Columba domestica)

H. K. N. AL-Mhanna

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 52-59

Formalistic study elucidate that the larynx in the fourteen healthy indigenous male pigeons (Columba domestica) for benefit in the study of the respiratory physiology, histopathology, and the respiratory diseases analyzes. After bird's preparation, the larynx detected, and then the shape, position and its components studied in details.The larynx emerges in the caudal part of the oropharyngeal cavity as a heart-shaped cartilaginous mass. It composed of a single hyaline cricoid cartilage which consisted of body and left and right wings, double hyaline arytenoid cartilages which consisting of body and rostral and caudal processes, and single hyaline procricoid cartilage which consisted of body dorsally and curved tail caudoventrally. These cartilaginous components surrounded by laryngeal skeletal muscles intrinsic (superficial and deep) and extrinsic (rostral, caudolateral, and caudomedial).

Treatment of Inactive Ovaries in Cows and Buffalos Heifers using a variety of Hormones

D.J. Khammas; N.A.K.M. Ali

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 60-65

This study was conducted on twenty nine cows heifers aged between 15-20 months and twenty two buffalos heifers aged between 20-30 months in Abu-Ghrabe and Al-Thahab Al-Abiadh villages at Baghdad suburban. On palpation all of the animals were suffering from inactive ovaries in addition to a history of an-estrum. Heifers of cows & buffalos were randomly divided into 4 groups and treated as follows G1 include 9 cows heifers and 7 buffalos heifers injected with 1000 I.U.(eCG) PMSG. I.M. (Folligon, InterVet). G2 include 6 cows heifers and 5 buffalos heifers injected I.M. with 0.5 mg GnRH analogue (Fertagyl, InterVet). G3 include 7 cows heifers and 5 buffalos heifers injected I.M. with 1000 I.U. PMSG and re-injected with 0.5 mg GnRH after 24 hr. G4 include 7 cows heifers & 5 buffalos heifers injected I.M. with 1000 I.U. PMSG and re-injected with 1500 I.U. hCG (Corulon, InterVet) after 24 hr. All the treated animals were left free with sires to ensure mating.Results of cows heifers responded & showed signs of estrus were 8/9 (88.8%), 4/6 (66.6%), 7/7 (100%) and 6/7 (85.7%) in G1, G2, G3 & G4 respectively. While the durations of response were 6.56±2.16 days, 7.22±3.52 days, 5.60±2.25 days and 6.74±2.18 days in the same sequence of groups. The number of heifers which subsequently became pregnant were G1 = 7/8 (87.5%), G2 = 2/4 (50%), G3 = 5/7 (71.4%) & G4 = 4/6 (66.6%).While the number of buffalos heifers responded to the same treatments & showed signs of estrus were 5/7 (71.2%), 2/3 (40%), 3/5 (60%) & 3/5 (60%) in G1, G2, G3 and G4 respectively. The duration of response were 6.14±2.06 days, 7.17±2.55 days, 5.93.±2.12 days and 5.95±2.02 days in the same sequence of groups. The number of the buffalos heifers which subsequently became pregnant were G1= 3/5 (60%), G2 = ½ (50%), G3 =3/3 (100%) & G4 = 2/3 (66.6%).

Histopathological And Immune Response Against Infectious Bursal Disease In Chickens Vaccinated Against Newcastle Disease

A.Z.AL Zubeedy; A.A. Shamaun; A.M.Al-Aalim

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 66-70

Two different vaccination programs against chicken infectious bursal disease (IBD) were compared. Results showed the first program including IBD vaccination at 8 and 16th day old as well as ND vaccination(live attenuated and inactivated types ) give high antibody titers at 21 days post vaccination with significantly difference (P≤0.0005) in comparison with the second program including only IBD vaccine . serological antibodies titer were determined to study the correlation between two different programs using ELISA test. pathological changes in vaccinated groups reveals mild & moderate lesions appear in liver, spleen and bursa of fabricius.

Pathological changes inducing by S.aureus in immunodeficiency mice immunized with Soluble Culture Filtrate S.aureus Antigens (SCFAgs).

H. H. K.AL-Byatee; M. J. A.AL- Jubory

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 71-81

In order to determine the influence of Soluble Culture Filtrate S.aureus Antigens (SCFAgs) on S.aureus infection in Mitomicin c immunosupression mice, seventy four white mice, both sex,7-8 weeks age were divided randomly into five groups.1st group(n=16 ) was immunized with 0.4ml of S.aureus CFSAgs (concentration of protein( 4.2mg/ml) ,i/p two doses, 2 weeks intervals. 2nd group(n=16) was injected with mitomycine C ,(1mg/kg B.W) I/p three time /week for 4 weeks. 3ed group (n=16) was immunized with CFSAgs as 1st group and treated with mitomycin as 2nd group. 4th group(n=10) was inoculated with (0.4ml) I/P with1X109 CFU/ML of viable virulent. S.aureus and was served as control positive group. 5th group (n=16) was inoculated with 0.5ml sterile normal saline. Cellular and humoral immune response were recorded at 28-30 day post immunization, skin test and passive heam agglutination test respectively, then all animals of immunized and treatment groups were challenge with S,aureus as control positive group. The results explained that animals treatment with MMC were died during (18) hrs post inoculation with virulent viable S.aureus with very heavy bacterial isolation, animal of control positive group were died at( 24)hrs post infection with heavy bacterial isolation The results revealed that immunization with CFSAgs elicited both humoral and cellular immune responses, the level values of both arms of immune response were lower animal treatment with MMC, Severe pathological lesions were seen in examined organs of control positive group but these lesions are more extensive in animal treatment with MMC. The main lesions in examined organs of these animals are suppurative inflammation ,congestion ,apoptosis and necrosis.. We conclusion that MMC induce immunosuppression condition and immunization with CFSAgs can improve the immune responses in the animals that are suffering from immunosuppression.

Cloning and gene expression equine leukocyte α-interferon in cells of Escherichia Coli

A. A. Muttar; M.I. Potapovich; V.A. Prokulevich

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 82-86

Interferon’splays role in innate immune responses through upregulation of costimulatory molecules and induction of proinflammatory cytokines.interferons including interferon alpha (IFNA). The present study characterized IFNA cDNA and predicted protein.The interferon’s play a great role in protection from infections, which have been called by microorganisms, and also have powerful antiproliferation and immunomodulation activity.The purposes of study:cloning andexpression of horse leukocyte interferonand purification the product protein. The results and discussion : In the result we isolated (DNA) from equine leukocyte in blood, which was using in the quality of matrix for amplification of α-interferon gene with PCR HELP, and isolation gene α-interferon and transformation in vector puc18 and expression vector PET24b (+) and recombinant plasmid was transformed into E. coli strain BL21( codon plus 440) induction with IPTG.The results showed the protein having the same molecular weight as horse interferon alphaabout 18.5 kDa.

The role of local propolis extract against harmful effects of acrylamide on some male reproductive parameters in rats

A. M. Ghazi; H. Kh. Ulaiwi; S. H. Jary

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 87-95

Propolis is a natural resinous substance obtained from bee hives living on various plant sources. The present experiment was designed to investigate the protective role of ethanolic extract of local propolis against the possible reproductive harmful effects induced by acrylamide when administered orally in male rates. A total of Thirty sexually mature male albino rats were randomly divided equally into three different groups (n = 10) , the first one : served as normal control , the second group received acrylamide alone (150 mg / Kg BW) , the third group received acrylamide in same dose and treated with local propolis extract (200 mg / Kg BW) twice daily. Doses were given by drenching for four weeks. The result of study revealed that administration of acrylamide induced significant decrease in the sperm concentration , sperm motility , rate of viability and normal sperms as well as decrease in weights of testes , epidydemis ,prostate gland , seminal vesicles , serum testosterone , FSH , LH levels with significant increase in sperm abnormalities compared with control group. Treatment with local propolis extract improved the harmful effects of acrylamide on reproductive parameters in male rats toward the normal values.

Study of the inhibitory effect of Ethanolic extract of( Quercus robur ,Cinnamomum zeylanicum and Thymus vulgaris ) on the growth of Staphylococcus aureus isolated from clinical mastitis in cow

Z. A. Hatem; J. A. Sabeeh

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 96-105

The present study was designed to evaluate the inhibitory effects of three local plant ethanolic extracts (Quercus robur , Cinnamomum zeylanicum and Thymus vulgaris) against the growth of Staphylococcus aureus isolated from milk of cow infected with clinical mastits in culture media by using of agar well diffusion method. For this purpose graduate concentrates for each extract (50 , 100 , 200 , 400 )mg/ml prepared and tested .The result showed that the extract of Quercus robur was more effective followed by Thymus vulgaris and Cinnamomum zeylanicum.The statistical analysis by using ANOVA with LSD at level (p<0.05) showed that there was no significant differences between the effect of the studied concentrations of Quercus robur , Cinnamomum zeylanicum while for Thymus vulgaris we find that the concentration 100 mgml had a significant difference with the other studied concentration in inhibition of the growth of the tested bacteria. We also find that between the antibiotics the largest zone of inhibition was given by LOM followed by SPV,PI,NV,RA and CX.

Study of some minerals deficiency in grazing sheep in Thi-Qar province.

I. A. Mohammed; J. A. Gadi; Amery; M.A.Y. Al; I. A. Mohammed

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 106-112

This study was carried out on 250 locally sheep collected from four sides of Thi-Qar Governorate. The clinical, hematological, trace elements changes in naturally occurring minerals deficiency in sheep were described. Anemia & Pale mucous membranes, Alopecia &steely wool, Diarrhea, parakeratosis, pica and abortion constituted the main signs and symptoms. The respiratory and heart rates were significantly higher (p<0.05) in mineral deficient sheep than in normal control sheep in one side of province. Values of total erythrocyte count, hemoglobin concentration and packed cell volume were significantly lower (p<0.05) in mineral deficient sheep than in normal control sheep. Significant differences were not found in total leukocyte count and differential leukocyte count values in sick and normal control sheep. Copper, Zinc and Iron values in the serum were significantly lower (p<0.05) in mineral deficient than in normal control sheep. The erythrocytes morphology appeared a abnormal shapes and size, this refer to Macrocytic hypochromic anemia and Normocytic hypochromic anemia, It was concluded that a significant changes were noticed between diseased and control sheep in clinical hematological values and trace elements and abnormal erythrocytes morphology, deficiency of a single element seldom occurs under field condition in Thi-Qar Governorate.

Eye worms, Thelazia gulosa (Railliet and Henry, 1910), In Buffalo, In IRAQ

Anisimova E. I; M.A.A. Al-Fatlawi; F.J. Al-Shammary

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 113-120

We reported 21 infections with Thelazia gulosa, in buffalo, slaughtered at Al-Diwaniya, Najaf and Babyl abattoirs, from john to September 2012.Eyes were examined carefully after uprooted 2 eyes from 328 buffalos for searching about parasite. By gently manipulating, the eyes checking in the conjunctival sacs and corneal surface.6.4% of examined eyes were have eyeworms.Diwania province showed higher infections rate, than Najaf and Babyl 10.22 %, 6.42% and 3% respectively. The worm burden arranged from 1-3 per eye, with mean number 1.8 parasite eye.According to months, September showed highly infections rate compared with June, July and August 18%, 2.38%, 2.22% and 0% respectively.In 12 buffalos we found 1 eye infect with eyeworm, and 9 with 2 eyes.

The molecular epidemiology of rotavirus strains causing gastroenteritis in infant in the region of Mid Iraq

W. S. Abood; S. A. Al-Juboury; M. A. Al-Rodhan

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 121-127

Rotavirus diarrhea is a leading cause of child death. It is a major concern in developing and developed countries. In Iraq, few studies and analysis were executed for genetic assessment of rotavirus.The aim of this study was to estimate the prevalence of rotavirus caused gastroenteritis and identify the distribution of the circulating G and P types of rotavirus among infants admitted to the hospital or treated as outpatients in three governorates in the region of Mid-Iraq for symptoms of gastroenteritis.This study was undertaken during the period from June 2010 to April 2012 in infants under 1 year suffering from acute gastroenteritis. Group A rotaviruses were detected in stools by latex agglutination test and genotyped using semi nested multiplex reverse transcription PCRs with type-specific primers on the basis of their outer capsid proteins.Of the 348 stool specimens from infants with acute gastroenteritis, 42.45% were positive for rotavirus A. The predominant G type was G1 (48.57%), followed by G2 (22.14%), G9 (11.42%), G3 (2.14%), G4 (0.71%). Only P [8] (61.4%), P [4] (11.4%) and P [6] (5.7%) genotypes were found. Infants of 9 and 10 months of age were most frequently affected. The prevalence of rotavirus infection peaked in the Autumn season, when temperatures were low, and decreased in summer. Epidemiological knowledge of rotavirus is critical for the development of effective preventive measures, including vaccines. These data will help to make informed decisions as to whether rotavirus vaccine should be considered for inclusion in Iraqi National Immunization Program.

Udder preparation and its effect on udder cleanlenees and milk quality

A. K. AL- Hubaety; R. H. Awad; R.I. THanon; F.A.Ez-Aldin

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 128-138

Total bacterial count was performed in eighty milk samples and eighty udder swabs collected from 20 cows, to compare the effect of treating udders with wet towel washing (55-60C0), followed by paper towel drying; three concentrations of Iodophre and Hibitane followed by paper towel drying and two concentrations of potassium permanganate followed by paper towel drying with non-treated udders .Results showed that Log10 of CFU/ swab of untreated udders was significantly (p≤ 0.05) differ from those swab samples when udder was treated with wet towel washing (55-60C0), followed by paper towel drying. By using iodophor, there was in asignificant (p≤ 0.05) reduction in the Log10 of CFU/ swab after udder treatment with all used concentrations of iodophor, and there was also proportional reduction in the Log10 CFU/ swab with each increase in the Iodophor concentration (0.1:100, 0.5:100 and 1:100) respectively. The same picture was gained by using Hibitane at concentrations of 0.25%, 0.5% and 1% as Iodophre. Potassium permanganate treatments with its two concentrations showed significant differences in Log10 /swab of udder before and after treatments and between its two concentrations 0.5:1000 and 1:1000.Total bacterial count of milk samples were a mirror of swab samples. From results it is evident that chemical disinfectants or hot water were effective in reducing udder contamination before milking.

Seroepidemiology of PPR in goats in Basrah province

R. K. Muhsen

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 139-143

This study was conducted in Basrah at period from March throughout the September 2011. 1175 blood samples were collected from goats in five regions of Basrah province that have no signs of disease and no any history of vaccination to estimate the seroprevalence of PPR in goats and undergoes to the cELISA test. The results of the study revealed that , the seroprevalence of the disease was 27.6 % . There were no significant differences in the seroprevalence in relation to sex and regions of the study (P ˃ 0.05 ). On other hand, the prevalence of PPR was higher significantly in the goats between 1 – 3 years of age compared with other age groups.

Separation of Y-chromosome Bearing Ram’s Sperms using an Albumin Gradient Technique and Identification of Embryos by PCR

S. Hadi; I. H. Al-Timimi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 144-151

Several advantages have been suggested for producing sexed sperms including using fewer and genetically superior female animals for replacement.Four hundred active ovaries collected from the slaughter house of Al-shu'alah, the number and type of oocytes, ratios of maturation and fertilization shown that there was a significant difference in the numbers of oocytes (P<0.05) between right and left ovaries. A high recovery rate was obtained of good oocyte (Grade A) 42.35% (432/1020), fair oocyte (Grade B) 37.54% (383/1020) followed by and poor oocyte (Grade C) 17.84% (182/1020). There was a significant difference (P<0.05) between the 3 different grades. grades A and B oocytes, (815/1020) 79.9% of recovered oocytes were cultured. Maturation rate was 86.38% (704/815).Y- Bearing sperms separation applied by using procedure of the modified albumin technique; either one (8%) or two layers (8 and 16%) of BSA (M1, and M2) at 200, 300 or 400 xg, then used for in vitro fertilization.The in vitro fertilization rate observed was 21.8% (132/604) of matured oocytes by choosing universal primers from sequences that are highly conserved in the X and Y chromosomes, sex-specific sequences were successfully amplified in embryonic lysates. Bovine serum albumin sexed sperms result in more percentage of male embryos by using one layer of BSA ( 8%) at 200 × g (M1a) and 300× g (M1b) which were 72.7% and 54.5% respectively, and shows a deviation (p<0.05) from the 50% expected percentage for male and female embryos. While using two layer of BSA (16% and 8% BSA) at the 200 × g (M2a) and at 300× g (M2b) were 81.8% and 63.6% respectively. When we compare the rate of male embryos produced from IVF by sperms isolated by two layers of BSA (M2a, and M2b), moderate results obtained with M2b (63.6%) while the best results were with M2a separation protocol (81.8%).

New parasites of local duck recorded in Iraq with histopathological study

N. Q. M. Al-Labban; Kh. A. Dawood; Gh. A. Jassem

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 152-161

This study was conducted to determine endoparasites in local ducks in Al-Diwaniya province. A total of 80 birds were collected from different areas of Al-Diwaniya city. Examination of the internal organs and fecal samples revealed the infection of (47.5%) of total examined birds with parasites. These parasites involved three specieses recorded as first time in Iraq which were the nematode Hystrichis tricolour with percentage of infection (10%), trematode Tracheophilus cymbium with prevalence (7.5%) and protozoa Wenyonella philiplevinei (3.75%). Many histopathological changes were noticed in the internal organs due to parasitic infections. The histological sections from proventriculas show irregular arrangement of cells and increase the empty spaces among the cells of glands, also sections from lung show caseous necrosis and cellular oedema full most alveoli in the lung with infiltration of inflammatory cells and mucous discharge in the lumen of bronchioles, in cecum histological section reveal destruction of villi with desquamation of epithelial cells lining villi and infiltration of inflammatory cells (eosinophil) in sub mucosa.

Failure to detect Helicobacter pylori in tap water sources in Al- Najaf and Babylon provinces by using PCR based on ureB gene.

O.M.Hadi; R. H. Fadel; H. K. Hussein

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 162-171

(500) sample collected from the study area, divided into (250) from the province of Najaf and (250) from the province of Babylon, and distributed by two models for each sample. I use the first model to estimate the level of free chloride , pH, dissolved substances college and temperature, while the use the other form of the same sample to check for the presence of bacteria in tap water in a PCR study showed a large disparity in the levels of pH, free chlorine, dissolved substances college and the temperature you two provinces were the highest percentages for the Abbasia in Najaf Ashraf and the least in the Alhaidariya in the same province study also demonstrated the existence of significant correlation between the level of free chloride each of the temperature, dissolved substances College and the pH while the highest in the region of Mahaweel in the province of Babylon, and the least in Jordan in the same area of the province and which should be mentioned to him the failure mode (PCR) in detecting the presence of Helicobacter pylori bacteria in tap water in the both provinces .
H.pylori :- Helicobacter pylori .
T D S :- Total dissolved solids .
PCR :- Polymerase chain reaction

The effect of Alcoholic plant (tribulus terrestris) extract on the bacteria isolated from genital tract of infertile cows in vitro

M.S.Qasem; A.M.Al-shawii; L.Y. A.Al Rahman

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 172-162

Out of Two hundred swabs collected from genital tract of infertile cows during the period between7/1/2012 to 30/6-2012. Cultured on Blood agar, Nutrient agar, MacConkey agar and Mannitol salt agar. 195 swab represented (97.5%) give positive result and 5 swab (2.5%) give negative result. from the positive culture 78 isolated (39%) mixed bacterial infection. All bacteria isolates were identified by using different biochemical tests and API-20E, the single bacterial isolation in this study were Esherichia coli 41.8%, Staphylococcus aureus36.8%, Pseudomonas aeruginosa17%, Citrobacter braakii1.7%, Providencia rettergi1.7% and Shewanella putrefaciens 0.9%. In mixed bacterial isolation, the highest percentage was 53.8% Staphylococcus + Esherishia coli aureus and the lowest percentage was 1.3% Esherishia coli + Citrobacter braakii + Pseudomonas aeruginosa. Antibiotic susceptibility test were carried out on bacterial isolates using 10 single antibiotic discs of commonly used drug, the test showed that most bacterial isolated resist for most antibiotics (Amikacin, Amoxicillin, Nalidix acid, (Amoxicillin and Clavulanic acid), Cefixime, Ceftriaxone, Tetracycline, Nitrofurantoin, Norfloxacin and Gentamycin) on the other hand observed sensitive for alcoholic plant tribulus terrestris extract (fruit) in different concentrations

Progesterone and estrogen levels in Awassi ewes treated with vaginal sponges

A.A. Omar

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 12, Issue 1, Pages 183-186

This study was conducted on10 healthy Iraqi Awassi ewes that examined by ultrasonography technique, with ages between 2 to 3 years, presented at the farm of the College of Veterinary Medicine, Al-Anbar University, Fallujah, during the period from the end of the February until the half of the March 2012. Vaginal sponges saturated with 40mg Flurogestone acetate were inserted in the vagina of each tested animals in order to induce estrus For synchronization for 14 days, at day 13of the experiment the animals were injected i.m. by Equine chorionic gonadotropine eCG (500 i.u.) then the sponges were removed and the animals injected i.m. with (200 i. u.) of Human chorionic gonadotropine (HCG). Blood samples were collected from jugular vein with vacutainer tubes each two days until the experiment was complete. Serum was collected after centrifugation with a speed 3000R/m for 10 minutes. Values of estrogen and progesterone hormones in serum were estimated by Enzyme Linked Immuno Sorbent Assay (ELISA) technique. Values of the estrogen and progesterone in the treated animals were (1.1932 ± 0.02380), (0.9558 ± 0.3282) respectively, while the levels of the two hormones for the control group was (1.1138 ± 0.02295) for the estrogen and (0.8702 ± 0.0152) for the progesterone. There was a significant different between the level of estrogen and progesterone hormones P < 0.05 in a treated group as a compare with the control group. It was concluded from this study that estrus synchronization of the ewes with vaginal sponge affected the values of estrogen and progesterone hormones in the serum.