Print ISSN: 1818-5746

Online ISSN: 2313-4429

Volume 10, Issue 1

Volume 10, Issue 1, Winter and Spring 2011, Page 1-135


Contamination of broiler's feed with aflatoxins in some broiler farms of Al – Diwaniya city

F.H. Abd-Allatif; K.N. Taher; M. A. Molaghi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 48-53

Contamination of broiler's feed with aflatoxins in some broiler farms of Al – Diwaniya city
M. A. Molaghi K.N. Taher F.H. Abd-Allatif
Coll. of Vet. Med. Coll. of agriculture

Abstract
This study was carried out to detect the contamination of broiler's feed with aflatoxins. A total of (42) different feed samples collected randomly from broiler's farms of Al- Diwaniya on 2 seasons , winter (Dec, Jan and Feb) and summer (May, June and July). (21) samples of each season. ELISA test was used for detection of contamination. The results showed occurrence of contamination with aflatoxin in (39) samples with percent (92.85%) and was only (3) samples (7.15%) free from aflatoxin. The contamination range between (0.5-140.3) part per billion (ppb) (µ/kg) with average mean (13.56) ppb , all samples of winter season were contaminated with aflatoxin (100%) while the contamination in (18) samples from summer season (85.71%). The quantity of aflatoxin in (7) samples (19.9%) a crossed the allowed limits determined by US-FDA.

Preparation of live attenuated vaccine of Streptococcus equi equi by submucosal injection in the upper lips of rabbits.

A.M.H.Al- Judi; M.A.Salih

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 1-3

Preparation of live attenuated vaccine of Streptococcus equi equi by submucosal injection in the upper lips of rabbits.
A.M.H.Al- Judi M.A.Salih
Abstract
Live attenuated vaccine of Strept. equi equi was prepared by culturing the bacteria in increasing concentration of acriflavin beginning from 2ppm to 16ppm by 77 passage for three months.The first group recived submucosal injection in the upper lip , and the second group recived intranasal installation of the vaccine. The titer of antibodies in the first group was 853.33 ± 107.93 8 weeks post vaccination, & 384 ± 57.24 in the second group with significant difference at the level of P< 0.05 between the two groups. Both groups did not show any clinical findings after challenge dose , while the control group died.

Analysis of chemical composition of raw milk in some farm animals in Al-Diwania city by using ultrasonic milk analyzer

F.H. Abdel-Lattif; K.N. Taher; K.H. Abbas

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 99-102

Analysis of chemical composition of raw milk in some farm animals in Al-Diwania city by using ultrasonic milk analyzer
K.H. Abbas K.N. Taher F.H. Abdel-Lattif
Coll. of Vet.Med./ Univ. of Al-Qadissia Coll. of Agri./ Univ. of Al-Qadissia
Abstract
This study was undertaken to knowledge the chemical composition of some farm animals ( cow,buffalo,sheep and goat ) by using ultrasonic milk analyzer . This method was used first time in our country in the feiled of milk hygiene to determine and comparsion the composition percent of raw milk for farm animals as attempt to protect the health of consumers . 240 raw milk samples for each species of animals included in this study for two different season of year ,first (Novembe 2009 - January 2010) , second from (May – July 2010) . The chemical analysis from milk samples for cows, buffaloes, sheep and goat show the percentage of composition as follow : Fat percentages were 3.7% , 6.8%, 3.4% and 8.7 % ,respectively. Solid non fat compounds (SNF) percentages were 8.6 % , 10.8 % , 9.3% and 8.7 % ,respectively.Protein percentages were 3.3 %,5.9 %, 5.5 % and 3.6 % ,respectively .Lactose percentages were 4.6 % ,4.4 % , 4.3 % and 4.3 % ,respectively . Season of year had a significant effect on percentage of milk composition .

The Effect of Alcoholic Extract of Citrullus colocynthus in Testicular Function of Adult Male Albino Rats.

B. D. I. Al-Wataar

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 90-98

The Effect of Alcoholic Extract of Citrullus colocynthus in Testicular Function of Adult Male Albino Rats.

B. D. I. Al-Wataar
Coll. of Vet. Med./ Univ. of Mosul

Abstract
The present study conducted to determine the effect of different doses (100, 200 and 300 mg / kg of body weight) of alcoholic extract of bitter melon in the functions of the testis. The results of the current study, showed these doses have caused a decrease in concentration of the testosterone compared with the control group, as well as the increase in the level of cholesterol, in addition to gross changes an increaseing in the size andcongestion of testis from mild to severe, compared with control group, in addition to necrosis and destruction in the cells of Leydig cells, which ranged also from the simple to the broad and destroy the spermatocytes and sperm and desquamation in the lumen of seminephrous tubules, and these lesions suited with dose given to male rats. Conclude from this study that the dose of 300 mg/kg of body weight of alcoholic extract of bitter melon have had the biggest impact on the functions of the testis to have had the impact in a significant decrease in the concentration of the testosterone, and increase in the concentration of cholesterol, also an increase in weight of testis, with testicular congestion and severe destruction of leydig cells with necrosis and desquamation of spermatocytes and sperm in the lumen of seminepherous tubule.
Keyword: Citrullus, Testosterone, Cholesterol, Male rats.

Possibility of uing wter etract of grlic and isecticide cpermethrin in teatment

K.A. Dawood; Th.E.Al-Mahnaa; G.A. Jassem

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 42-47

Possibility of uing wter etract of grlic and isecticide cpermethrin in teatment

K.A. Dawood Th.E.Al-Mahnaa G.A. Jassem
Coll.of Vet.Med. /Univ.of AL-Qadisiya

Abstract
The effect of water extract of garlic on hard ticks in comparison with cypermethrin by using spray method in vitro and in vivo. The result of this experiment reveals that 250 mg/ml water extract the most effective concentration on ticks than other concentrations, while 1mL/L of cypermethrin more effective than garlic extract with significant variation of probability (P<0.05). The toxicity of garlic was examined on the back of rabbits histological and pathological changes were studied.

Prevalence of liver fluke and lungworm among slaughtered animals in Al-Najaf abattoir

K.Z.Khalil

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 11-17

Abstract
117908 head of ruminant slaughtered at Al-Najaf abattoir from 1/1/2009 to 30/12/2009, were examined for Liver fluke and Lungworm prevalence. The study samples include( 60430 sheeps , 31375 goats ,19430 cattles , 2526 buffaloes and 4147 camels ).The incidence rate of Liver fluke was 0.962% in cattles , 0.593% in` buffalos , 0.630% in sheeps , 0.372 % in goats , and there is no infection in camels .While the incidence rate of Lungworm was 0.174% in cattles , 0.168 % in sheeps ,and 0.242% in goats, but no infection recorded in buffalos and camels. The highest infection rate with liver fluke in cattles, buffalos, sheeps was in autumn in a rate of 1.906%,1.507%,1.153% respectively ,while in goats was in winter(0.684%).while the lowest rate was in cattles, buffalos,& goats was in springs 0.04% ,0.169 % ,0.025 %,while in sheeps in winter (0.06%) .The study also show that highest infection rate with Lungworm of cattles, sheeps , & goats was in winter (0.467%, 0.266%,0.101%)respectively. The lowest rate of them was in summer(0.038 % , 0.103 % , 0.023 %).

Draw the Epidemiological Map of FMD in Iraq According to the Official Records

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 54-66

2011 Draw the Epidemiological Map of FMD in Iraq According to the Official Records
Abstract
This study was conducted to draw the epidemiological map of FMD in Iraq at 2006,2007,2008 and the first semester of 2009 according to the data of State Company of Veterinary Service Minstry of Agriculture, and Kurdstan Territory .That show centralize of FMD in infection in province that adjacent to neighbor countries which FMD were recorded in it , and the animal movement led to spread the disease to another provinces. And also revealed the highest prevalence percentage in cow in the first semester of 2009 about 21.59 % and the highest prevalence was recorded in AL Basrha province as 8.5 % . The highest prevalence of disease in sheep was recorded at 2007 as 4.074% and highest percentage was determined in AL Basrha province (3.150 %) . In August ,the highest prevalence percentage of FMD was showed in cattle ,while in sheep was recorded at June as 0.026% ,0.566% in 2008 respectively . In AD Diwaniyah province ,the highest prevalence percentage of FMD infection was recorded as 0.253 %,0.119 % in Cow and sheep respectively . In AL Najaf province ,the prevalence of FMD in buffalo was recorded as 0.904 % at the first semester of 2009, and the highest morbidity rate was showed in April in the same year .

Study the histological changes in kidney by different doses of vitamin C in rabbits

S. D.A. Al-Gnami; I. A. Al-Athary

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 4-10

Study the histological changes in kidney by different doses of vitamin C in rabbits
S. D.A. Al-Gnami I. A. Al-Athary
Coll. of Vet. Med. /Univ. of Al-Qadisiya
Abstract
This study carried out in animal house in medicine collage in al-kofa university ,there are 32 local rabbits were used which ranged between(11-12) months in age, and (1100-1600) gm in weight ,all rabbits was femal only.The animals were divided into four equal groups , each group contain 8 animals, the first one leaved as control and the others drenched by 7,14 and 21 mg of vitamin C for each Kg.B.W. daily for 3 weeks, and the management conditions are similar in all groups.The results of the microscopic study show the presence of many of the histological changes as a result of treatment with vitamin and found dilation of renal tubules and small glomeruli when dosage the animals with 7 mg / kg of vitamin C, as results showed the sever dilation in the renal tubules with the survival of glomeruli are normal, as well as to the presence hemorrhage in the kidney tissue at the dosage of animals with 14 mg / kg of the vitamin, as the results indicate the emergence of necrosis and expanded greatly in tubules kidney for females with deposit of hyaline with vitamin C concentration of 21 mg / kg with the appearance of cloudy swelling in renal tubules and the lumen appear star shape, stars with get destroyed in the glomeruli.

Comparative study of histopathologic changes associated with administration of five different Gumboro disease vaccines

B.H. Ali; E. J. Khammas

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 82-89

Comparative study of histopathologic changes associated with administration of five different Gumboro disease vaccines
B.H. Ali E. J. Khammas
Coll. of Vet. Med./ Univ. of Baghdad

Abstract
Two hundred and four broiler chicks have were divided into six groups, each group inoculated a vaccine as follows: (G1) BUR-706, (G2) IBD-L, (G3) TAD, (G4) CH/80, (G5)D78 and (G6) considered as control group without any treatment. Histopathologic examination was carried out on Bursa of Fabricius (BF), spleen, thymus, Harderian's gland and cecal tonsils. Score lesions of BF were determined and Apoptosis score of cells was estimated using Ag-NOR silver nitrate stain. The pathological changes observed 24 hours post vaccination were interfollicular edema in the BF with depletion of lymphocytes in the follicles and congestion hemorrhage, also, depletion of lymphocytes in spleen, thymus, Harderian's gland and cecal tonsils. Bursal lesion scores (BLS) showed disappearance of negative impact of the vaccine gradually. Apoptosis test was done on the less affected groups (G1 and G2) showed significant increase in the cells.

The inhibitory efficiency of flavonoid extract of black mustard seeds (Brassica nigra) in growth of some bacteria and fungi isolated from local and Imported beef carcasses

H. H. Hashem

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 67-77

The inhibitory efficiency of flavonoid extract of black mustard seeds (Brassica nigra) in growth of some bacteria and fungi isolated from local and Imported beef carcasses

H. H. Hashem
Coll.of Vet. Med./ Univ of AL-Qadisiya
A. H. AHamadani
Coll of Med./ Univ of AL-Qadisiya
I. F. Al–Jawhary
Coll of Med./ Univ of AL-Qadisiya

Abstract
This study was conducted to detect the microbial numbers and species that associated the local and imported beef carcasses and the sanitary conditions for the slaughterhouses and the butcher’s shops in Al-Diwaniya city, and the possibility of illness germs existence that are connected with the food poisoning and gastroenteritis cases in human, like Staphylococcus aureus , Escherichia coli and other fungi like Penicillium notatum, Aspergillus spp. and others, in one hundred and sixty samples of beef carcasses (80 samples of local meat and 80 samples of imported meat) . The results showed that the total count of aerobic bacteria and Fungi for the beef carcasses when results statistically analysis under possibility level (P<0.05) show indicated of low significant differences between the local and imported meat , the local meat was more contamination than the imported meat , while the fungal contamination in imported meat was more than the local meat . Isolation and Identification of bacterial species from the local and imported meat and from the swabs which taken during this study , were Staphylococcus aureus (46.30 % in the local meat & 37.88 % in the imported meat ) , Escherichia coli (41.94 % in the local meat & 43.17 % in the Imported meat ) , Pseudomonas aeruginosa (9.73 % in the local meat & 12.77 % in the Imported meat ) and Proteus spp. (2.01% in the local meat & 3.96% in Imported meat) , while the most common fungi were Penicillium notatum (62.83% in the local meat & 73.77% in the Imported meat) , Aspergillus niger (24.77% in the local meat & 11.47% in the Imported meat ) , Alternaria alternata (7.07% in the local meat & 9.83% in the Imported meat) ,Aspergillus candidas (4.42% in the local meat ) , Aspergillus fumigatus (0.88% in the local meat & 3.27% in the Imported meat) and Aspergillus spp. (1.63% in the Imported meat ).The Flavonoid extract of Black Mustard seeds (Brassica nigra) show inhibition activity in growth of the following bacteria (Staphylococcus aureus , Pseudomonas aeruginosa) and fungi (Penicillum notatum , Aspergillus niger , Alternaria alternata ) and in all concentrations that uses (25,50,75,100) mg/ml , while Escherichia coli showed susceptibility in the concentration (75,100) mg/ ml and the results showed differential in the extract activity according to Microbial kinds that uses in this study that belong to species of bacteria and fungi and its structure. The Minimum Inhibition Concentration (MIC) of extract have been determined and in bacteria was (30) mg/ ml for each S. aureus and Ps. aeruginosa and (40) mg/ml for E. coli .and the Minimum bactericidal Concentration was (70)mg/ml for all bacteria which examined , while in fungi the minimum Inhibition concentration was (0.25) mg/ ml and the Minimum fungicidal Concentration was (5) mg/ml for all examined fungi

Detection of Fascioliasis in sheep and cattle by using of ELISA technique

A.M.A. Al-Khafajy

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 131-135

Detection of Fascioliasis in sheep and cattle by using of ELISA technique
A.M.A. Al-Khafajy
Coll. of Vet Med./Univ. of Al-Qadissiya
Abstract
The aim of present study detection of Fascioliasis in sheep and cattle immunologically in dewania city by using ELISA test. The results showed high percentage of seropositive sheep and cattle as 76.7% and 74.5% respectively .
The results of this study revealed an association between the percentage of infected animals and age , so the cattle with age more than and less than one year give 82.3% and 17.6% respectively while the sheep in the same above ages recorded 44.1% and 55.8% respectively .

Study of the chemical and quality characteristics of frozen ground beef in Baghdad retail storages

D. A. A. Al-Obaidi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 23-29

Study of the chemical and quality characteristics of frozen ground beef in Baghdad retail storages
D. A. A. Al-Obaidi
Coll. of Vet. Med./ Univ.of Baghdad
Abstract
This study was carried out at the College of Veterinary Medicine, University of Baghdad, for three month's, aiming to study the shelf life of frozen ground beef of retail storage's in Baghdad by evaluation some chemical and quality characteristics. The data obtained revealed the following result's: Haaila mark of ground beef had the highest percentage of moisture (76.1%), with lowest percentage of lipid, ash, carbohydrates, and protein. Awal mark of ground beef had the highest percentage of lipid ranged (6.3-6.6%),with lowest percentage of protein, carbohydrates and ash. Ahul Al beadier mark of ground beef had highest percentage of carbohydrates especially for Babb Al-muddam region retail storages (4.48%), with lowest percentage of lipid, ash, and protein. Quality test indicated that thawing loss percentages were high, specially in Ahul-al beadier and Haaila marks which were (9.4%) for Al-mansour region retail storages. Myoglobin concentration was low for all marks of ground beef specially Haaila mark of ground beef for Dourra region retail storages were 111mg / 100g meat, T.V.N., F.F.A., P.V. and T.B.A. test values were in the allowance limits for Iraq specification of ground beef. The results indicated that necessary to make routine chemical checking and evaluation to keep a good nutritional value of the ground beef for consumer then the safe public health.

Effect of vitamin E on some haematolgical aspects in cold-stressed broiler chickens

H. H. Alewi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 36-41

Effect of vitamin E on some haematolgical aspects in cold-stressed broiler chickens
H. H. Alewi
Technical Institute/ Al-Musaib
Abstract
The present experiment was conducted to evaluate the effect of vitamin E in modulating the side effect of cold-stress in broilers. Sixty (21 day old) Ross broiler chicks were randomly divided into two equal groups (2 replicates, 15 for each). All chicks were exposed to low environmental temperature (13°C) for 12 hours / day (06:00 p.m. to 06:00 a.m.) for 7 days. At the same day, tap drinking water were used for control group, and the drinking water for treatment group supplemented with Vitamin E (α-tocopherol) at a concentration of 15 I.U.L of drinking water.Results revealed that supplementation the drinking water of birds with Vitamin E resulted in significant decrease in heterophil / Lymphocyte (H/L)ratio (P<0.05), and blood glucose (P< 0.001) as compared with control group. The body weight of vitamin E treatment group were significantly ( P< 0.01) higher than control group. There was no significant difference between the two groups in haemoglobin (Hb) and packed cell Volume (PCV). The mortality rate was lower in vitamin E treatment group (3.33 %) as compared to control group (10 %).Results of the present study indicate that vitamin E supplements attenuate the negative effect of low environmental temperature by decreasing H/L ratio , blood glucose, and mortality rate while increasing body weight in cold-stressed chickens.

Some microbial contaminates in frozen fish in local markets of Diwanyia city

O. M. AL-KWZAIY

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 18-22

Some microbial contaminates in frozen fish in local markets of Diwanyia city

O. M. AL-KWZAIY
Coll. of Vet. Med. / Univ. of Al-Qadisiya
Abstract
The study have shown The effect of Storage periods on frozen fish which imported to Iraq which sale in local markets of diwanya city which imported from two origins(Menmar and Gorden ).(60)samples were collected (20)sampls from each origin in three storage periods (two monthes ,two monthes and two weeks ,three monthes ).the study shown Total aerobic bacterial count ,Total coliform bacteria ,staphylococcus aureus count ,proteolytic bacteria and determined the rates some types of Aeromonas species . Total aerobi1c bacterial count were (1.59 × 510 cfu /g , 10 5 × 1.88 cfu /g 2.27 ×510 cfu /g) in Menmar and in Gorden (1.05 × 10 5 cfu /g, 1.31 × 510 cfu / g ,1.84 × 510 cfu /g) . Total coliform bacteria in Menamar were 10 4 × 0.85 ) cfu /g, 1.07 × 410 cfu /g, 1.20 10 4 × cfu /g)and in Gorden fish were ( 0.32 × 410 cfu /g, 1.20 × 410 cfu /g, 2.04 × 410 cfu /g) . staphylococcus aureus count were (1.15 × 10 3 cfu /g , 1.41 × 10 3 cfu /g, 1.86 × 10 3 cfu /g) in Menmar and in Gorden fish were (1.58 × 10 3 cfu /g, 1.85 310 × cfu /g,1.90 × 10 3 cfu /g) , proteolytic bacteria count in Menmar were 2.15 × 10 3 cfu /g , 2.24 × 10 3 cfu /g, 2.98 × 10 3 cfu /g) and in Gorden were(1.02 × 10 3 cfu /g, 1.26 × 10 3 cfu /g, 1.54 × 10 3 cfu /g).The rates of A.salmonicida in Menmar was 2.1% and in Gorden fish was2.6%.the type A.caviae in Menmar WAS 1.8% and in Gorden 0.0% but the type A.sobria in Menmar fish was 3.8% and in Gorden fish was 2.2% .the type A.hydrophila in Menmar fish was 0.0% but in 1.2% .

Investigation of brucellosis in female buffalo in Nassiria City by using Rose Bengal

A.F. Daham

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 78-81

Investigation of brucellosis in female buffalo in Nassiria City by using Rose Bengal

A.F. Daham
Coll. of Vet. Med. Univ. of Al-Qadissiya
Abstract
The current study was designed to investigate prevalence of brucellosis in buffalo in Nassiria province by using Rose Bengal Test. (84)samples of serum of female buffalo were examined. The results revealed that (5) cases (5.95%) of total samples were positive and distributed according to age into three groups: (3-6),(6-9) and (12-15) years (1 out of 26 samples),(3 out of 45) and (1 out of 3) in percent of (1.19%), (3.57%) and (1.19%) respectively. The highest standard of antibody titration was (1/32) appears in the first group (3-6) years. All positive cases suffered meteritis or abortion.

Antifertility potential of Juniperus phoenicea fruits extract in mature female rats

A. H. Saliem; F. R. Jaafar; M. A. Al-bayaty

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 114-120

Antifertility potential of Juniperus phoenicea fruits extract in mature female rats

M. A. Al-bayaty A. H. Saliem F. R. Jaafar
Coll. of Vet. Med./ Univ. of Baghdad

Abstract
The plan of this study was to determine the effects of alcoholic extract of Juniperus phoenicea fruits on the estrous cycle, ovulation, fertility and fetal morphology of cyclic mature Sprague-Dawley rats. Mature female Sprague-Dawley rats, weighing between 180-240gm were used. There were three main experimental groups. Group one rats received 1 gm/kg B.W. of alcoholic extract of Juniperus phoenicea fruits by oral gavage for 30 days (5 estrous cyles) and the effect on estrous cycle studied.Group 2 rats were administered 1 gm/kg of Juniperus phoenicea fruits alcoholic extract at 8 a.m. and at 5 p.m. on proestrus and the effect on the number of ova shed on the morning of estrus checket. Rats in Group 3 were treated with 1 gm/kg BW of alcoholic extract of Juniperus phoenicea fruits on days 1 to 5 postcoitum, and observation was completed for anti-implantion / abortifacient effects and their teratogenic effects on the fetuses. All the groups were control-matched. The estrous cycle of 80% of the rats was altered with a marked prolongation of the diestrus phase. Juniperus phoenicea fruits caused a statistically significant (p< 0.05) reduction in the number of ova shed in the morning of estrus in rats treated with the extract at 9 a.m. on proestrus. Neither anti-implantion / abortifacient nor teratogenic effect was observed in the rats treated with Juniperus phoenicea fruits. Treatment of alcoholic extract of Juniperus phoenicea fruits disrupted the estrous cycle in mature rats and caused a partial block in ovulation and thus has the potential of being developed into a female contraceptive.

Histopathological study of Effects of continuous injection of dexamethasone on wound healing in rabbits

F. Salim

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 1-7

Histopathological study of Effects of continuous injection of dexamethasone on wound healing in rabbits
F. Salim
Coll. of Vet. Med./ Univ of Basraha
Abstract
In this study 16 rabbit was divided in to two groups equally the first group was daily injected by dexamethasone(1 mg/Kg) for 21 days,the second group was left without any treatment as a control. An excision was made in the skin of rabbits and then sutured with silk band 2.5cm.The skin spicemen were taken for histopathological sections for wound healing evaluation at postoperative days 3,7and 14. The scars of the dexamethasone-treated group were less formation. The epithelization ,collagenization and the inflammatory cell infiltration was less intense in the dexamethasone- treated group in compare with the control animal.

Microbiological study of Otitis externa in Sheep in Diwaniya City

S.A.Ali

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 32-35

Microbiological study of Otitis externa in Sheep in Diwaniya City

S.A.Ali
Coll. of Vet.Med. /Univ of Al-Qadisiya
Abstract
In this study, twenty eight Awassi sheep suffering from otitis externa were examined for isolation and identification of bacterical infections.The study showed 53.6% of the sheeps were positive for bacterical islates.The results showed that Pseudomonas aeruginosa was the most common bacteria (39,1%) followed by Staphylococcus aureus (26%) , Mannheimia haemolytica and Staphylococcus epidermidiis (13%) for each,while E.coli and Streptococcus spp. were less in common (1%) for each. Sensitivity tests of these isolate were studied and show highly sensitivity to ciprofloxacin and norfloxacin and highly resistant to Ampicillin.

Histopathological study of Balb/c organs infected with Candida albicans isolated from Buffaloes in Basrah province

B. A. Abbas; I. S. Touhali; M. H. Khudor

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 95-103

Histopathological study of Balb/c organs infected with Candida albicans isolated from Buffaloes in Basrah province

B. A. Abbas M. H. Khudor I. S. Touhali
Coll. of Vet. Med. /Univ of Basrah

Abstract
Experimental infection of Balb/c mice with the locally isolated C. albicans from buffaloes blood sample at dose 1× 106cells/mice Intra-peritoneum was investigated. The dose was found to be sufficient to cause infection and induced pathological changes among the tested mice organs. The organs which included were brains, lungs, spleens, livers, kidneys and uterus were affected by yeast and showed variable types of lesions.

Embryonic development of nasal septum in indigenous sheep

S. Kh. M. J. Al-Taee

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 77-83

Embryonic development of nasal septum in indigenous sheep

S. Kh. M. J. Al-Taee
Coll.of Vet. Med/ Univ. of Mosul

Abstract
The study included detection the onset time of the chondrification and ossification of nasal septum in indigenous sheep fetuses. Fetuses have been prepared by using stained with modified double staining method in young ages and maceration with potassium hydroxide in older ages. First sign of nasal septum demonstrated as small spot of condensed mesenchyme of medial nasal prominence of Meckel's cartilage (Hyaline cartilage). Chondrification process of nasal septum initiated at 54 day old. The primary ossification centre of the caudal part of nasal septum appeared at 61 day old in the distal caudal end of mesenchyme of Meckel's cartilage. The examination of nasal septum growth from sheep embryos in the (42-63 days) period shows that this is a rapid period of growth is in the end of the first trimester and the first of second trimester of gestation. The increase in the length of the nasal septum is about three folds (from 0.5 cm at 7 week to 1.6 cm at the 10 week of indigenous fetal age. The results of analysis of variance done on the relative increase in the nasal septum length and nasal septum height showed that the greatest relative increase in nasal septum length was at the 7 week, while the greatest relative increase in the nasal septum height was in the 11 week.

Efficiency of Naphalene degradation by Pseudomonas bearing Nah-Plasmids in model soll system

F.J.H. Al-Shammri; Titok

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 121-130

Efficiency of Naphalene degradation by Pseudomonas bearing Nah-Plasmids in model soll system
М.А. Titok F.J.H. Al-Shammri
Belarusian State University Coll. of Vet Med./Univ. of Al-Qadissiya

Abstract
A large collection of naphthalene-degrading Pseudomonas strains isolated from pollu-tion sites was screened for the growth parameters in the liquid medium with naphthalene, the utilization of organic compounds (single and complex as petroleum) as the only source of carbon and energy, the activity of key enzymes of naphthalene catabolism (naphthalene diox-ygenase, salicylate hydroxylase, catechol-1,2-dioxygenase and of catechol-2,3-dioxygenase). The most active strains were used as recipients of Nah-plasmids. The transfer and inheritance of Nah-plasmids of IncP-7 and IncP-9 groups in the cells of natural naphthalene utilizing bac-teria and collection strains of Pseudomonas were studied. The efficiency of naphthalene deg-radation by natural and genetic constructed strains bearing Nah-plasmids in model soil system was determined. The number of microorganisms and the concentration of naphthalene in soil samples were monitored (in presence of bacteria the concentration of naphthalene in soil has been decreasing from 32,2 % to 100 % in 7 days). The best protective effect was found for the collection strain P. putida KT2442, bearing Nah-plasmids from group IncP-9 and naturals bacteria P. putida AL21, P. putida NL21, P. putida NL26, P. putida AL1 containing combi-nation of two Nah-plasmids. These strains can be used as a basis for biopreparations for effec-tive cleaning polluted soils
Key words: naphthalene, naphthalene dioxygenase, salicylate hydroxylase, catechol-1,2-dioxygenase, catechol-2,3-dioxygenase, plasmid, model soil system.

A comparative study between using of Midazolam-Ketamine and Diazepam-Ketamine combinations as anaesthetic program in sheep

S. A. A. Al-Redah

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 66-72

A comparative study between using of Midazolam-Ketamine and Diazepam-Ketamine combinations as anaesthetic program in sheep
S. A. A. Al-Redah
Coll. of Vet.Med./ Univ. of AL-Qadisiya
Abstract
The present study was designed to compare the efficacy for using of the two anesthetics combinations (Midazolam-Ketamine and Diazepam-Ketamine). The effect of combinations on the length of surgical anesthesia, degree of flank analgesia, degree of muscle relaxation, respiratory rate, heart rate and rectal temperature were assayed in sheep.The experiment was conducted on ten apparently healthy adult sheep of either sex weighting (24-33kg). Sheep were divided in two equal groups, Diazepam (1mg/kg BW) +Ketamine (10mg/kg BW) group (D+K), Midazolam (1mg/kg BW) +Ketamine 10mg/kg BW group (M+K), was used by intramuscular injection, benzodiazepine derivative (diazepam or midazolam) injected first and then followed by ketamine 10minutes later.The following parameter were recorded at 3minutes before injection of drug and 5, 10, 20, 30, 45, 60 and 75 minutes (min.) respectively after administration of anaesthetic drug (Rectal Temperature , Respiratory Rate , Heart Rate , Degree of Analgesia , Degree of Muscle relaxation), the induction time, surgical anaesthesia and recovery time also were recorded.The experiment showed that the anaesthesia with Midazolam + Ketamine combination in sheep was suitable compared with Diazepam + Ketamine as it produced reliable surgical anaesthesia, good analgesia and muscle relaxation.

The effect of aqueous extract of Marticaria Chamomilla flowers on some physiological properties in broiler chickens.

S. A. H. AL-Moramadhi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 59-65

The effect of aqueous extract of Marticaria Chamomilla flowers on some physiological properties in broiler chickens.

S. A. H. AL-Moramadhi
Coll. of Vet. Med./ Univ of AL-Qadissiya

Abstract
This study was conducted on broiler chickens to investigate the effect of Marticaria chamomilla flowers extract on some physiological properties. One day age Fawbro birds (Average weight 55g) were used in this study, they fed ad libtum until 7weeks age. (60 chickes) were divided into three groups (each group has 20 birds ).
1-treatment one: administrated orally the aqueous extract of Marticaria chamomilla flowers at concentration 50mg /kg body weight .
2-treatment two: administrated orally the aqueous extract of Marticaria chamomilla flowers at concentration 100mg /kg body weight
3-control group: administrated distilled water .At the end of experiment blood samples were taken from brachial vein for ten birds of each group for hematological and bio chemical examination ,the following parameters are used:- serum glucose concentration, serum cholesterol concentration ,hemoglobin concentration ,packed cell volume ,body weight and food intake. Results showed significant decrease (p<0.05) in glucose concentration in treatments groups compared with control, and significant decrease (p<0.05) in cholesterol concentration in treatments groups compared with the control. While there was no significant effect on hemoglobin concentration, packed cell volume, body weight and food intake in treatments groups compared with the control.

Physiological effects of Roselle calyces extract in some blood parameters in laying hens

R. J. T. Al-Baghdadi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 54-58

Physiological effects of Roselle calyces extract in some blood parameters in laying hens
R. J. T. Al-Baghdadi
Coll. of Vet. Med./ Univ of Al-Qadisiya
Abstract
This study was designed to investigate the physiological effects of alcoholic extract of Calyces of Roselle (Hibiscus sabdariffa) in some blood parameters in laying hens. 30 layers, 49 week old belonging to the Isa Brown breed were randomly divided into two groups of 15 layers each, both are received basal diet. Group 1 served as control which was orally administered 2ml/kg body weight of distilled water. Group 2 served as treated group which was orally administered alcohol extract of Hibiscus sabdariffa calyces at a dose of 200mg/kg BW dissolved in 2 ml of distilled water. The groups received the water and extract daily to individual layers at 6:00 o’clock in morning, after that drinking water and feed were provided ad libitum, the experiment lasted 8 weeks from 25/12/2009 until 25/2/2010. The effect of the extract was assessed by estimating red blood cells count, Hb concentration, white blood cell count, and serum: total protein, cholesterol, LDL and HDL, The results revealed that Roselle calyces extract produced significant increase in the levels of Hb conc., white blood cells and HDL, and significant decrease in the level of LDL, while the numbers of red blood cells and levels of total serum protein & cholesterol had non significant elevation in Group 2 compared to Group 1.

Study of Some Physiological and Pathological Aspects of Feral Queen’s Reproductive System in Iraq

T. A. Abid; D. H.Jassim; H. J.Kadhim

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 14-25

Abstract
The present study is carried out to investigate some aspects of reproduction in Iraqi feral cats. Sixty blood samples and female genital specimens from sexually mature queens were trapped during the period from December 2008 to December 2009.During the entire study period, the results showed that incidence rate of follicular phase was 24.56 % and the highest incidence was in Winter 62.5%, while no follicular activity was noted in animals during Autumn . The hormonal assay showed that the mean of the estradiol -17ß in 14 follicular phase animals was 47.5pg/ml, while The dominance of vaginal epithelial cells in 14 estrus queens were nucleated superficial cells 61.5 %.The incidence of luteal phase was 29.82% and the highest incidence was in Spring 56.66%,and the average of corpora lutea per animal was 4.7 % and the incidence rate of pregnant queens was 19.29%,with high incidence in Summer 33.33% wih no pregnant queens was captured in Winter. The implantation rate was 86.06% and the transuterine migration of embryos was observed in incidence rate 27.27% and all transuterine migration of embryos was occurred in animals with 100% implantation rate . The maximum number of embryos that migrate was 2 /animal. The incidence rate of pseudopregnancy 11.76%, and characterized by present of active corpora lutea on ovaries , with slight enlargement of uterus and uterine tube .
In all animals with diestrus the mean of plasma progesterone level was 48 ng/ml ,and the cell comprising the vaginal smear include intermediate cells 47% parabasal cells45% and nucleated cells 5% .The anestrus phase was recognized in incidence rate 36.84% and the highest incidence of anestrus phase was noted in Autumn 75%, Cells comprising the vaginal smear in anestrus queens include intermediate cells 87.9%, parabasal cells 8.6%,nucleated superficial cells 2.7% , anucleated superficial cells 0.8% .The pathological study was revealed 3 out of 60 queens with gross reproductive systems abnormalities ,with incidence rate 5% ,these cases include paraovarian cyst, follicular cysts and pyometra.

Hemato-biochemical and histopathological alterations induced by acute cypermethrin toxicity in rabbits.

H. Kh. Ulaiwi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 84-94

Hemato-biochemical and histopathological alterations induced by acute cypermethrin toxicity in rabbits.
H. Kh. Ulaiwi
Coll.of Vet.Med./ Univ of Al-Qadisyiah

Abstract
The present study was conducted to evaluate the acute toxicity of cypermethrin in rabbits. Twenty four rabbits were used in the study and kept under similar management conditions. After seven days of acclimatization, rabbits were randomly divided into two equal groups : the treated group (T) and control ( C ) group and the treated group was subdivided according to dose of administration of cypermethrin drug into 3 groups includes: T1, T2 and T3. The cypermethrin was administrated into treated group by oral administration of (15 mg/kg B.W.; 25 mg/kg B.W. and 50 mg/kg B.W.) to (T1, T2 and T3) respectively, while the control group( C ) was received normal saline. Rabbits in each group received three treatments at an interval of seven days. All the animals were monitored twice daily for clinical signs. The blood was collected from control and treated group to evaluate the following blood parameters: Hb rate, PCV%, RBC counting number, WBC counting number and platelet counting number; also the plasma was separated to evaluate total protein concentration. Two rabbits from each treated group was sacrificed to study histopathological alteration in stomach, intestine, kidneys and lungs at the last day of experiment. The result of this study revealed that the erythrocytes count were decreased significantly (P<0.05) in all cyprmethrin treated groups, compared to control group. The concentration of Hb decreased significantly (P<0.05) in (T2 and T3) groups at periods of (21) and the result showed significant differences(P<0.05) between (T3) and all other groups of study, while PCV decreased at day 14 in groups (T1 and T2) and at day 21 in group (T3) only, compared to control group, and the result revealed significant decreases(P<0.05) in group (T3) as compared with other groups. Total white blood cells counts were significantly (P<0.05) increased specially at (21) in group (T3). Platelets count showed significant decreased (P<0.05) in all groups at 14 and 21 days of experiment as compared with control groups and (T3) revealed significant differences (P<0.05) as compared with other groups. The result ensured that there were significant decreased (P<0.05) in total protein in the (T2) and (T3) groups at period of (21) day. Gross investigation of stomach revealed eccymotic hemorrhage in the stomach and engorgement of blood vessels. The results of histopathological alteration revealed that there are engorgement of pulmonary capillary and congestion of pulmonary alveoli, thickening and congestion of the alveolar septa and swollen and tortuous of small vein, the pulmonary alveoli are full of amorphous eosinophilic fluid. In duodenum there are necrosis and destruction most of the superficial glands, with present some viable glandular tissue. In kidney there are destruction of few renal glomeruli and deposition of eosinophilic materials in the renal tubules with dilatation of the tubules. The renal tubules severely dilated and full with eosinophilic material. The renal glomeruli completely atrophied and destructed.

Histopatholgical changes in the mice model of surgical unilateral cryptorchidism

Aula E. Hadi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 26-31

Histopatholgical changes in the mice model of surgical unilateral cryptorchidism
Aula E. Hadi
Coll. Of Vet. Med./Univ. of Basrah
Abstract
This study was done on 20 male mice (35 – 45) days with weight of (18 – 20) grams divided into four groups, each group contains five animals.The first group regarded as control and remain intact. The second, third and fourth group were subjected to experimental unilateral cryptorchidism to render them cryptochild by putting the testes inside the abdominal cavity and suture the inguinal canal.Second group were killed after 10 days from the operation, the third group were killed after 20 days & fourth group were killed after 30 days.This study confirms that intraabdominal cryptorchid testes are impaired spermatogenesis due to abnormal localization, decrease in testicular volume in the cryptorchid group, seminiferoustubular atrophy, basnet membrane thickening, germ cell loss and spermatogenic arrest were more evident with time being highest at the 30 days.

Diagnostic & immunological study on Aspergillus fumigatus of camels in Thi-Qar province

A.J. Na

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 73-76

Diagnostic & immunological study on Aspergillus fumigatus of camels in Thi-Qar province

A.J. Na’ma
Coll. of Vet Med./Univ. of Al-Qadissiya
A. I. Al-Ameed
Coll. of Vet Med./Univ. of Baghdad
M. H. Hussain
Coll. of Vet Med./Univ. of Al-Qadissiya

Abstract
Fungi have an important role as an etiological factor in respiratory tract disorders in farm animals. In camels, this study was conducted to discover the immune status against Aspergillus fumigatus ; the most common fungi in the world. 90 camels were inspected and the blood was sampled from herds in Thi Qar governorate. Camels ranked in different ages, 1-10 years, and both male and female were included. The study dialed with camels suffering respiratory disorder. Serum was examined by passive hemagglutination test using the antigen of Aspergillus fumigatus isolated and prepared by (1). 16 camels were positive which present 17.8% , this result informs an important role of Aspergillus fumigatus as a cause of respiratory tract disorder in Iraqi camels.
Introduction

Effect of Lawsonia inermis extract on the pathological changes of skin infection by Streptococcus pyogens in lab. Mice

E. A. H. Kadhum; B. M. M. Al-Mehna

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 45-53

Effect of Lawsonia inermis extract on the pathological changes of skin infection by Streptococcus pyogens in lab. Mice

B. M. M. Al-Mehna E. A. H. Kadhum
Coll. of Vet. Med. Univ of Al-Qadisiya

Abstract
In this study, the antibacterial effects of aqueous and alcoholic extracts of Lawsonia inermis leaves against Streptococcus pyogenes were investigated in vitro by using agar well diffusion method and in vivo using laboratory mice by treating it with prepared ointment from these extracts & compared the effects wih Gentamicin . Results showed that both extracts demonstrated antimicrobial activity against the tested organism but the efficiency of the extracts was significantly affected by the solvent used in the extraction as well as the concentration of extract. Alcoholic extracts had the highest antibacterial activity it exhibited an inhibition zone 18.2-28.2 mm in comparison with 26.2 mm for gentamicin .A prepared ointment from alcoholic extract in a concentration 5% was tested for treatment of the experimentally infected skin of mice by S. pyogenes using scratching . The recovery period was 11 days in compared with 10 days for gentamicin. Biopsy was taken from the infected area to detect the pathological effects occurred by tested bacteria ,also biopsy was again taken after the treatment with the prepared ointment to evaluate the anti-inflammatory ,antibacterial and wound healing activity of Lawsonia inermis. Histopathological study showed after 3days from skin infection with Streptococcus pyogens hypremia, swelling, congestion and pus formation also abscessation While in treated animals with henna extracts gross skin lesions disappeare after 11 days from infection and on day 10 showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells when compared with the controls which showed inflammatory cells, scanty collagen fibres and fibroblasts.

Toxicologic pathology study of diclofinac in rats by oral intubation

A. H. Sadoon; A. A. Ibrahim; S. K. Majeed; M. A. Ramadhan

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 104-113

Toxicologic pathology study of diclofinac in rats by oral intubation
S. K. Majeed A. A. Ibrahim A. H. Sadoon M. A. Ramadhan.
Abstract
Aim of the study:- This study aimed to evaluate the toxic effect of the diclofinac since this drug is one of the most common non steroidal anti inflammatory drugs which is widely used as a pain relief for several pathological cases.
Materials and methods:- Rats were kept in the animals house for two weeks to get acclimatization then treated with 25 mg/kg/day and 50 mg/kg/day as intermediate and high dose respectively by oral intubation daily for three monthes to show the toxic effect of the drug.
Results:- Heart showed vacoulation of myocardial muscle cells, liver showed degenerative changes and periportal fibrosis.But the more severe toxicological pathological changes were in the kidney as dilated bowman's space with fluid distention in glomeruli, degenerate/ necrotic cortical mineralized tubules and changes in medullary collecting ducts. The above changes were more severe in high dose than in intermediate dose which was considered as dose related changes.
Conclusions:- diclofinac has a toxic effect on several organs in the body if it is use for long period even within the normal therapeutic doses.

Effect of Treated Genital Diseases on the Number of Services per Conception in Dairy Cattle

T. Gh. M.Ali; I. H. Al-Timimi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 8-13

This research is applied on small private fields of lactating cows located in Sulaimania region. One hundred fifty four lactating non pregnant cows involved in this study, from which 31 cows (20.13%) suffered from uterine infections, 41 cows (26.63%) were with ovarian problems and 82 (53.24%) were clinically normal cyclic cows. Cows suffered from genital disorder were treated according their reproductive state.All clinically normal cows and cows responded to treatment were observed for detection of estrous and inseminated artificially, and number of services per conception (SPC) were recorded. Number of services per conception (SPC) for clinically normal cyclic cows was 2.0±0.10.In our study, we reported for the first time, that SPC were 1.0±0.00, 1.2±0.20 and 1.7±0.15 for cows treated from luteal cystic ovaries, inactive ovaries and persistence corpus luteum respectively, and the Mean ± S.E. of SPC for this category of cows was (1.54±0.15), While a very high SPC were necessary for cows treated from uterine infections; 4.0±0.24, 4.4±0.25 and 5.0±0.45 were recorded for cows treated from endometritis, toxic puerperal metritis and metritis respectively, and the Mean ± S.E. of SPC for this category of cows was (4.39±0.34).

Evaluation of the inhibitory effect of a number of medicinal plants fixed oils on a group of pathogenic bacteria invitro

Z. A. H. Al- Ebady

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 10, Issue 1, Pages 36-44

Evaluation of the inhibitory effect of a number of medicinal plants fixed oils on a group of pathogenic bacteria invitro
Z. A. H. Al- Ebady
Coll. of Vet. Med./ Univ Al- Qadisiya

Abstract
The present study was designed to evaluate the antibacterial effect of 4 of local medicinal plants oils :Eletteria cardaomum,Nigella sativa,Prunus dulcisvar and Brassica alba in addition to 5 Antibiotics: Ciprofloxacin ,Chloramphenicol, Aztereonam, Sulfamethaxazole /Trimethoprime and Erythromycine as a positive control against 7 gram positive and gram negative pathogenic bacteria:Micrococcus spp.,Staphylococcus cohnii cohnii ,Enterobacter cloacae,Escherichia coli, Proteus mirabilis,Pseudomonas aeroginosa and Klebsiella pneumonia by using of agar well diffusion method . The result showed that for the oil of Eletteria cardamomum the best antibacterial effect was produced against Proteus mirabilis followed by Klebsiella pneumonia then Enterobacter cloacae,Pseudomonas aeroginosa,Escherichia coli,Micrococcus spp. and Staphylococcus cohnii cohnii , and for the oil Nigella sativa the antibacterial effect was highest against Micrococcus spp., followed by Proteus mirabilis then Escherichia coli and Staphylococcus cohnii cohnii. Brassica alba oil express it is antibacterial effect only against Pseudomonas aeroginosa and there was no effect on any other bacteria while Brassica alba had no effect on any of the studied bacteria.The inhibition of the growth of Pseudomonas aeroginosa was stronger by Prunus dulcisvar oil than Eletteria cardamomum oil .For Escherichia coli,Staphylococcus cohnii cohnii and Micrococcus the wider zone of inhibition were obtained by Nigella sativa oil than Eletteria cardamomum oil, while for Proteus mirabilis the inhibition of the growth was higher for the oil of Eletteria cardamomum than Nigella sativa oil.The values were given as mean ±Standard deviation and P< 0.05 was considered statistically significant.The datad were analyzed by student's t-test using SPSS (version 10).