Print ISSN: 1818-5746

Online ISSN: 2313-4429

Volume 9, Issue 2

Volume 9, Issue 2, Summer and Autumn 2010, Page 1-84


Effect of different types of RBCS and temperature on haemagglutination of NDV

A. Z. Daewd

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 1-4

Effect of different types of RBCS and temperature on haemagglutination of NDV

A. Z. Daewd

Coll. of Vet. Med. / Univ. of Mosul

Abstract
The effect of different types of red blood cells and temperature on haemagglutination of Paramyxo that cause Newcastle disease virus ( vaccinal strain) Lasota was studied. Results showed that the titer of vaccinal strain in haemagglutination test with red blood cells of chicken, turkey and (O) blood group of were 1:64 after 30 minutes and 1:16 in Cattle , sheep and rate through (45) minutes , In horse and buffalo 1:4 after 90min . The effect of temperature on haemagglutination of Newcastle dense virus with chicken red blood cells showed the titer 1:64 in room temperature at 28°C and 1:8 in refrigerator temperature at 4°C but give the titer of 1:16 incubator temperature at 37°C .

Epidemiological Study of caprine sarcocystosis in Babylon province

F. S. kadihm; M. H. Mohammad

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 5-15

Epidemiological Study of
caprine sarcocystosis in Babylon province

M. H. Mohammad F. S. kadihm
Coll. of Vet. Med./ Univ. of Babylon

Abstract
The study was aimed to investigate the prevalence of macroscopic and microscopic sarcocystosis in 428 goats ( male = 215, female = 213) slaughtered in Babylon province abattoirs.The macroscopic examination used to detected macroscopic cysts in carcasses and the microscopic examination (peptic digestion method, trichinoscopy, squeezing and histological examination) used to detect the microscopic cysts exists in esophagus, skeletal muscle, diaphragm and heart. The results were appeared macroscopic type infestation in goats with S. caprifelis reach 0.93 %. all the infection were found in esophagus with 0.93 % and didn’t appear in the other organs. we were found two types of macroscopic cysts which are fat & thin ones, it’s appear spindle or oval diffusing and embedding in muscular fibers of the infestation organs. The macroscopic cyst bradyzoites characterized by crescentic shape.The microscopic type S. capracanis in goats was 93.22 %, the percentage were variable depending on methods of examination, the higher percentage was found in peptic digestion method was 95.99 % then squeezing method was 91.22 % fallowing by trichinoscopy was 84.48 % and finally the histological examination method was 36.59 %. The microscopic cysts examinated by trichinoscopy appeared were in different shapes, it was oval, ellipsoidal, cylindrical and cone shaped and divided into locules interactive with each other contain the microscopic cyst bradyzoites which appeared as crescentic shape and have pointed anterior end and rounded posterior end in peptic digestion & squeezing methods.There were two type of bradyzoites, the first type was long thin arching and second type was the short fat and less arching, in addition to that the microscopic cysts appeared two different shapes by histological examination method which the first shape with thin wall and the other have thick and transversally striated wall.

Chemical qualitative and bacterial assessment for imported canned corned beef in Diwaniyah city

B. G. M. AL-Grabi; A. L. D. AL-Hisnawi; H.G.H. AL-Khauzai

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 16-22

Chemical qualitative and bacterial assessment for imported canned corned beef in Diwaniyah city

A. L. D. AL-Hisnawi H.G.H. AL-Khauzai B. G. M. AL-Grabi
Coll. of Agr./ Unive. of Al-Qadyssia Coll. of Vet. Med./ Unive. of Al-Qadyssia
Abstract
The current research was aimed to study some chemical, quality changes in addition to the know the level of bacterial contamination for imported canned beef to Iraq in Diwaniyah city .The results of chemical analysis appeared that meat of Lebanese origin with Hana mark had a highest dry matter and fat percentage which were 44.35% and 19.475% respectively compared to other meat, while meat of Jordanian with Almaraaia mark had a highest protein and ash percentage were 14.925% and 5.025% respectively compared to other meat, and the highest percentage of carbohydrate was recorded for meat of Brazilian origin with Creat cow mark and was 5.425% .Also qualitative assessments, which included total volatile nitrogen were conducted and the highest value of its was for meat of Brazilian origin with xtrah mark was 13.100mg/100g of meat moreover Brazilian meat with xtrah mark recorded a highest average for Thiobarbituric acid 0.92 malonaldehid /kg meat the results revealed that amount of dye myoglobin was 168.500mg/100g of meat as the highest value in Almarraaia mark .On concerning bacteriological test the result were within limits allowed according to international standard where the number of total anaerobic bacteria ranged from 0-7 X 110 /gm of meat and the average of the numbers of Clostridium bacteria was 0-5 X 110 /gm of meat .

Effect of retained placenta in some blood characteristics in Holstein cows

M. L.AL-Taee; A. H. AL-Mahdawi

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 23-27

Effect of retained placenta in some blood characteristics in Holstein cows
M. L.AL-Taee A. H. AL-Mahdawi
Abstract
Records of 600 Holstein cows were analyzed statistically to investigate the factors that effect the incidence of retained placenta. Blood samples were collected from 40 cows with retained placenta to estimate calcium, magnesium and phosphorus that associated with the incidence of retained placenta.Result of the present study showed that parity, season of calving, sex of calf, type of calving and level of milk production, were the most factors might cause or increase the incidence of retained placenta in Holstein cows. Blood levels of calcium, Magnesium and phosphorous significant decrease (7.82, 4.89, 4.66 mg/ml respectively) compared to normally expelled placenta (9.15, 6.39, 5.18 mg/ml respectively).

Evacuation study to hygienic state of local cheese and compared with processed cheese which product from Al-Qadisiya diary factory

O. Meteb; F.Hassen; A.Abd Rahim

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 28-32

Evacuation study to hygienic state of local cheese and compared with processed cheese which product from Al-Qadisiya diary factory.

F.Hassen O. Meteb A.Abd Rahim
Abstract
A total of (80) samples of two types of cheese were collected (local cheese (40) and processed cheese (40)) during May-December of 2005 .The Microbial examination showed in crease their micro organisms content:-Means of total bacteria numbers in local and processed cheese were (7.2×108 )CFU)gr, 2.8×106 )CFU)gr) respectively .Means of coliform bacteria numbers were (6.6×106 )CFU)gr, 2.6×105 )CFU)gr) res.Mean of streptococcus (4.95×105 )CFU)gr, 2.9×104 )CFU)gr) res.Means of staphylococcus aureus numbers (8.6×105 )CFU)gr, 4.2×104 )CFU)gr) res.Means of psychotrophic bacteria numbers (8.6×106 )CFU)gr, 2.7×105 )CFU)gr) res.Means of thermphilic bacteria numbers (11.17×106 )CFU)gr, 3.0×106 )CFU)gr) res.Means of proteolytic bacteria numbers 5.7 × 106 )CFU)gr, 2.2×105 )CFU)gr) res.eans of liplytic bacteria numbers (5.1×107 )CFU)gr, 3.5×106 )CFU)gr) res.Means of spore forming b. numbers (4.8×106 )CFU)gr, 3.3×105 )CFU)gr) res. Means of molds and yeasts (6.0×105 )CFU)gr, 2.0×103 )CFU)gr) res. Salmonella isolates rate in local cheese was 57.7% and in processed cheese 5% this study emphasized that the processed cheese manufactured in more hygienic conditions than local cheese.

Study the incidence of abortion and retained placenta in pregnant cows with ephemeral fever and its relation with treatment and bacterial causes

M. Y. Obeed; H. R. Abass; H. A. Abdul Hameed

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 33-37

Study the incidence of abortion and retained placenta in pregnant cows with ephemeral fever and its relation with treatment and bacterial causes

H. A. Abdul Hameed H. R. Abass M. Y. Obeed
Coll. of Vet. Med. Unive. of Bassra
Abstract
This study was conducted on (335) pregnant cow which infected with ephemeral fever disease.(238) cow was treated with antibiotic and antipyretic (187 cow multipara and 51 unipara) , and (97) cow was left with out treatment (69 cow multiparp and 28 cow unipara) when the signs of disease appear .Than the percentage of abortion and retained placenta estimated in two groups ( treated and non treated ) and also estimated the total abortion percentage according to pregnancy months. And also subjected to bacteriological study on secretions of aborted fetus to excluded the specific and nonspecific bacteria combined with abortion cases.The results of this study revealed the incidence of abortion in treated cow in ratio ( 10.5%) and (14.43%) in untreated cow; also the results showed that most abortion cases were occurred in the late three months of pregnancy especially in 8th month of pregnancy in ratio (41.02%) from the total cases of abortion.The study also showed the occurrence of retained placenta in treated and untreated aborted cows with ratio (72 , 85.71%) respectively.The results of bacterial study of aborted fetus secretions revealed that the rate of positive bacteriological isolation was (100%) from the total aborted cases ; the nonspecific bacteria formed the most of the bacterial isolation ,and recoded that the E.coli , Streptococcus spp. and Klebsiella spp. the highest rate of isolated microorganisms.

Study and comparative the chemical composition of some type of hens eggs which used in Diwaniya city

Z. A. Al. Zuhairy

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 38-42

Study and comparative the chemical composition of some type of hens eggs which used in Diwaniya city
Z. A. Al. Zuhairy
Coll. of Vet.Med./ Univ.of AL-Qadissiyah
Abstract
The aim of this study was study and compare the chemical composition of yalk and albumin in four types of hens which are use in Diwaniya city, White leghorn adapted in Turkia, Iasbrown adapted in Iraq, Iraqi strain, Isabrown adapted in Syria. The chemical characterizes included percentage of moisture, ash, protein, lipid, and carbohydrate of yalk and albumin, and cholesterol, high density lipo protein(HDL), LDL in yalk egg. The result obtained of significant increase (P<0.05) of ash and protein in Turkey white leghorns and Syrial isabrown compared with another types, and there is no significant difference in lipid, moisture, carbohydrate. In yalk found significant increase in protein, ash and HDL percentage in White leghorns and Syrial isabrown egg and we show significant increase in lipid and cholesterol in Iraqi strain and Iraqi isabrown compared with White leghorns & Syrial isabrown. This study indicate that the Turkey white Leghorn and Syrial Isabrown produce eggs similar in the chemical composition and contain high level of protein and low level of lipid and cholesterol than Iraqi strains. Thus strains produce probably safer eggs as far as cholesterol concern.

Pathological changes in livers of cattle infected with Helminthes in Babylon province

H. S. Al- Biaty

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 47-54

Pathological changes in livers of cattle infected with Helminthes in Babylon province
H. S. Al- Biaty
Coll. of Vet. Med./ Unive of Baghdad

Abstract
This study was conducted to characterize the pathological changes result from infection with both F.gigantica and hydatid cyst in livers of cattle slaughtered in Al Hella abattoir a total of 327 livers were examined during period from 1st march to 30th November 2008 .55 of these examined cattle were infected with F.gigantica and 51 were infected with Hydatid cyst while 17 of these infected cases were infected with both F.gigantica and Hydatid cyst that’s mean the infection of liver with one of these two parasite not prevent from infection with another and these cases were isolated from females with age of more than 4 years then took samples from livers which were with both infection for histopathological changes .Economical losses due to both infection was 26.300320 I.D.

Inhibitory effect of green tea extract on bacteria isolated from respiratory tract infections

M. S. Abbas; A. I. Modafer; I. J. Lafta

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 55-61

Inhibitory effect of green tea extract on bacteria isolated from respiratory tract infections

M. S. Abbas I. J. Lafta A. I. Modafer
Coll. of Vet. Med./ Unive of Baghdad
Abstract
The sensitivity of 20 gram-positive and gram- negative bacterial isolates obtained from patients suffered from respiratory diseases were studied. Gram- positive bacteria included: 4 isolates of both Staphylococcus aureus and Streptococcus pneumoniae and one isolate of Corynebacterium spp., while gram- negative bacteria consisted of Klebsiella pneumoniae (1 isolate), Klebsiella oxytoca (1 isolate), Escherichia coli (2 isolates), Pseudomonas aeruginosa (6 isolates) and Neisseria meningitidis (1 isolate). Camellia sinensis Linn leaf ethanolic extract antimicrobial activity was studied. We compared the mean zone of growth inhibition produced by green tea extract with that of the eight antibiotics used in this study. Green tea was effective against all tested gram- positive bacteria and some gram- negative bacteria, in which mean zone of growth inhibition of 14.66 mm in S. aureus, 14.5 mm in S. pneumoniae and 14 mm in Corynebacterium spp. was observed. As far as gram- negative bacteria, green tea extract produced the highest zone of growth inhibition of 15 mm against N. meningitidis and the lowest growth inhibition zone (8.5 mm) in case of E. coli and it was not effective in inhibition of P. aeruginosa, K. pneumoniae and K. oxytoca.The antimicrobial activity of green tea extract was similar to that of Neomycin when testing the sensitivity of N. meningitidis, while it was similar to oxytetracycline when testing S. aureus. The green tea extract gave low growth inhibition zones, but was higher than that reported by 5 antibiotics: (cefaclor, clindamycin, oxytetracycline, neomycin and cefotaxime) when studying S. Pneumoniae sensitivity. P. aeruginosa revealed wide antibiotic resistant, in which it resisted 7 antibiotics, followed by E. coli and S. aureus, as both of them resisted 6 antibiotics.

Identification of Buffaloes hemorrhagic septicemia in southern Iraq marshland-2008

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 62-68

Identification of Buffaloes hemorrhagic septicemia in southern Iraq marshland-2008
Abstract
A satisfactory investigation of buffaloes hemorrhagic septicemia was obtained in the marshes of Dhi Qar governorate in southern Iraq. The clinical and pathological symptoms and cultural, morphology of bacterial colonies and biochemical characters of Pasteurella multocida were described witch isolated from 13 samples that collected from infected and dead animals. Gross and histopathological changes were recorded. also in 13 samples collected from infected also in 13 samples collected from infected.

Using U.V. lights for improving of hydrocarbon degrader Pseudomonas aeruginosa D18

Z. T. Al-Sedrah; I. H. Gatie; H.R.R. Al-Taai

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 69-73

Using U.V. lights for improving of hydrocarbon degrader Pseudomonas aeruginosa D18
H.R.R. Al-Taai  I. H. Gatie  Z. T. Al-Sedrah 
 Coll. of Agr./ Unive Diyala  Sciences and Technology Ministry

Abstract
The U.V lights was used in different times (5-75) sec. to obtain mutant isolates that are capable of hydrocarbon degradation, the isolate could to reduce biosurfactant to 30mn/m.Study of plasmids content for selected isolate showed that its content 4 plasmids different in size.Bacterial conjugation was conducted; the results showed plasmids could not transfer through conjugation process.

Comparative study of some Serum Biochemical parameters of cattle and sheep of the marshes in the south of Iraq

Kh. G. Al-Fartosi; Y. J. Talib; Sh. Ali

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 78-84

Comparative study of some Serum Biochemical parameters of cattle and sheep of the marshes in the south of Iraq
**Kh. G. Al-Fartosi *Y. J. Talib * Sh. Ali
*Marshes researches center , Univ of Thi-Qar. ** Coll of Sci. Univ.of Thi-Qar
Abstract
This study includes the normal values of biochemical parameters of cattle and sheep lived in Marshes of south of Iraq .The serum levels of glucose, urea, creatinine, cholesterol ,triglyceride,total protein, albumin, globulin ,calcium(ca), potassium(k), Asparate amino transferase(AST),Alanin amino transferase(ALT), Alkalinephosphatase ( ALP) and Lactate dehydrogenase (LDH) were determined .The results showed there are non significant differences in all parameters of male cattle compared with female , except the serum glucose which decreased significantly in male compared with female. There are a significant increase in cholesterol and potassium of male sheep compared with female , whereas the other parameters did not show a significant difference (p<0.05) between them. also, the results indicated a significant increase in TG , cholesterol , AST , ALK and LDH of male and female cattle compared with male and female sheep. There were not significant differences in other parameters.
Key words: Biochemical parameters, Cattle, Sheep, Marshes of Iraq.
Corresponding author: khalidalfartosi@yahoo.com

The histological effects of vitamin A supplement on the vaginal epithelial cells during estrus and luteal phases in Awassi sheep

A. M. Hussin

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 69-77

The histological effects of vitamin A supplement on the vaginal epithelial cells during estrus and luteal phases in Awassi sheep
A. M. Hussin
Coll. of Vet. Med/ Univ of Baghdad.
Abstract
This study was carried out on the vaginal epithelium of eight Awassi ewes during estrus and luteal phases in order to evaluate the histological effect of vitamin A on the vaginal epithelium, vaginal smears were taken and stained by methylene blue. Light microscope was used in examination of slides. The present result revealed that vitamin A stimulated the process of keratinization which in turn raised the immune response of the vagina. The process of keratinization formed a sheet of strongly attached polygonal cells which lines the vagina. Moreover, the most striking conclusion from this study was the indication to the period of arrival of the neutrophils from the blood to the vaginal epithelium which lasts more than six hours. These observations indicate the importance of vitamin A in proliferation, keratinization and increase cellular immunity of the vagina.

An evaluation of the antifungal activity of some local medicinal plants against growth of Candida albicans in vitro.

A. M. G. Al-Mohana; J.S. Al-Hussaini

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 60-68

An evaluation of the antifungal activity of some local medicinal plants against growth of Candida albicans in vitro.
J.S. Al-Hussaini A. M. G. Al-Mohana
Coll. of Vet. Med. Unive. of Al-Qadisiya
Abstract
The aim of this study was to evaluate the antifungal activity of the ethanolic extract of three local plants ( Elettaria cardamomum , Aloe vera, Thyme vulgaris) against the growth of pathogenic Candida albicans in culture media. The antifungal activity was carried out by using agar well diffusion method. Ethanolic extracts of Elettaria cardamomum and Aloe vera inhibited the growth of Candida albicans isolates at all concentrations which tested in present study (25, 50, 100, 150, 200, and 400) mg / ml, while the extract of Thymus vulgaris showed no activity against tested Candida albicans

The activity of alcoholic extract of Garlic on the growth of Staphylococcus aureus with estimation of median lethal dose in lab. Mice

E. M. Rashid

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 53-59

The activity of alcoholic extract of Garlic on the growth of Staphylococcus aureus with estimation of median lethal dose in lab. Mice


E. M. Rashid
Med. Tech. Institute / Mansour
Foundation of Tech. Edu.
Sh. Jamal
Coll of Med./ Univ. of Baghdad

Sh. M. Al-khazraji
Med. Tech. Institute / Mansour
Foundation of Tech. Edu.


Abstract
The study was intended to investigate the in vitro activity of alcoholic extract of Garlic on the inhibition of growth of Staph. aureus which was isolated from skin infections , and determine the median lethal dose ( LD 50 ) of the extract in lab. mice. The Garlic was extracted by ethyl alcohol 95% , the ratio of ethanolic extraction amounted 44% of the weight of dry substance. Graduated concentration were prepared from alcoholic extract of Garlic from 10-100 mg/ml. Their activities were checked up against Staph.aureus by agar diffusion method using ethylene glycol as control. The results showed that the sensitivity of the test bacteria was gradually increased with increasing the extract concentration , the concentration 10-30 mg/ml were rather low active in preventing the growing of Staph. aureus in culturing media , the concentrations 40-70 mg/ml were moderately active ,meanwhile the concentrations 80-100 mg/ml were highly active against the growing of Staph.aureus . The results also showed that the LD50 of the ethanolic extract of Garlic when it is orally administered to the lab mice by gradual concentrations was about 8000 mg/kg body weight . The toxic signs during 24 hrs after initial feeding with the extract were rapid breathing followed by dullness , then death .

Characterization of E coli phage isolated from sewage

G.A. Al-Mola; I. H. Al-Yassari

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 45-52

Characterization of E coli phage isolated from sewage

G.A. Al-Mola I. H. Al-Yassari
Coll. of Uloom Al-Banat / Unive of Babylon
Abstract
Bacteriophage are viruses that infect bacterial cells. as with all viruses, phage are nonliving agents and thus require the use of the host’s metabolic processes to replicate itself. in this study, the phage of interest are those that infect and lyses E. colt host cells. when phage are released from the ruptured host, distinct zones of clearing (plaques) form. the original E. colt host cells for this experiment came from a sample of raw sewage. in order to obtain the bacteriophage, a procedure of enrichment, isolation, dilution and seeding was followed, the presence of distinct plaques indicated that lytic bacteriophage had been successfully amplified, separated and grown.This study included determination of phage titre, latent period , rise period and the burst size of the phage and effect some of factor on phage titre such as (temperature, ether and chloroform) .for determination ofhage titre used series of dilutions(10-1, 10-2, 10-3, 10-4, 10-4, 10-6, 10-7, 10-8, 10-9) the dilution factor gave the best countable number of plaques is(103). this dilution factor was then used for all other experiments, the latent period , rise period and the burst size of the phage are determined by countable number of plaques and phage titre(titer: plaque-forming unit(p.f.u) during 10,20,30,40,50, and 60 minutes . it was (4.7x105 ‘ 5.3x105 and 6.0x105)during 1O,20and30minutes respectively in the latent period ,but it was (8.5x105 8.9x10’ 9.3x105)during 40,50,and 60 minutes respectively in the rise period .then the burst size of the phage is counted by the ratio of the phage titer after rise period to that during the latent period it was(1.67).This study also included effect of temperature on phage titre the statistical analysis was significantly increase P<0.05 in phage titre at the temperature37 C° comparing with phage titre at the temperature 50 C° and phage titre at the temperature 65 C°. effects of ether and chloroform on number of plaques and phage titre during 5,10,15 ,20,25 ,30,35 and 40 minutes it was(0.7x105 , 0.3x105 , 0 , 0 , 0 , 0, 0 and 0) respectively in ether sensitivity, but the phage titre in chloroform sensitivity was completely inactivated by chloroform treatment, the statistical analysis (freedom degree ( 2,21 ) and F value=52.60 was high] significant increase (P<0.05) in phage titre in normal saline comparing with phage titre in ether and chloroform sensitivity.

Study induce Ectopic pregnancy by diaminobenzine in Rabbits

A. Sh.Aliawy

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 37-44

Study induce Ectopic pregnancy by diaminobenzine in Rabbits
A. Sh.Aliawy
Abstract
Five adult female rabbits ,their body weight is between 1.5-2 kg and their age ranges between 12-14 weeks along with 3 adult male for an probation .The animal housed in hygienic conditions at arrival immediately check for pregnancy were found negative (non pregnant). After that rod were inserted in vagina to induce puncture any part in uterus or uterine horn to administer 3,3′ Diaminobenziden .After 29 days agent test for pregnancy there were found pregnant .Blood samples examined for hormonal and enzyme changes and for counting of red blood cell (RBC) white blood cell (WBC) packed cell volume(PCV) and ham globulin (Hb).There were a significant changes found in the blood values profess values and on vial the values of progesterone.

Study of propofol-thiopental mixture for induction of general anesthesia in xylazine premedicated Donkeys

O.A. Bader; L.M. AL-kattan

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 31-36

Study of propofol-thiopental mixture for induction of general anesthesia in xylazine premedicated Donkeys

O.A. Bader L.M. AL-kattan
Coll. of Vet. Med./ Unive. of Mosul
Abstract
The current study was conducted to evaluate the anesthetic quality produced by Propofol-Thiopental mixture (P-T) in six donkeys premedicated with Xylazine (X), (1.1 mg/kg IV). The intravenous dose schedule for P-T mixture was 1, 2 and 4 mg/kg of Propofol with 5 mg/kg of Thiopental Sodium, respectively. Each donkey was anesthetized three times with each dose of P-T, five minutes after X administration in random order at one week intervals.The anesthetic parameters induction, duration of anesthesia, time to sedation and recovery times, cardiovascular parameters, respiratory parameters and incidence of side-effects were qualitatively and quantitatively assessed.The results of the present study revealed presence of wide range of differences in the quality of anesthesia between the three doses of P-T mixture. The high dose of P-T mixture (4 mg/kg+5 mg/kg) produced an ultra induction time (7±4 seconds), which was free of excitement with longer onsets of sleeping times (54 min). But, times to regain sternal and standing positions were longer with this dose. While, the low dose (1 mg/ kg+5 mg/kg) failed to produce narcosis. On the other hand, the (2 mg/kg+5 mg/kg) of P-T, produced smooth induction (17.5±3 seconds) with sufficient tracheal intubations. Time to narcosis was shorter than that produced by the higher dose (animal still recumbent) (19.1±4), but with rapid recovery time (25.8±5.6). The transient apnea observed in this study which was usually followed by administration of P-T mixture, was shorter (37.8±18 sec.) with the medium dose (2 mg/kg+5 mg/kg), compared to that with the higher dose.It could be concluded that P-T mixture (2mg/kg+5 mg/kg), is an excellent anesthetic protocol for induction of general anesthesia in donkeys. It is the first record for the use of this mixture for general anesthesia in this animal.
Key words: General anesthesia, Donkey, Propofol-Thiopental mixture.

Seroprevalence of Coxiella burnetii among cows and sheep in Thi-Qar province -Iraq.

J. Abed; A.A Salih; A. Abd-ul-husien

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 26-30

Seroprevalence of Coxiella burnetii among cows and sheep in Thi-Qar province -Iraq.

J. Abed A.A Salih A. Abd-ul-husien
Vet.hospital/ Thi-Qar Coll. of Nursing/Unive of Thi-Qar Coll. of Scie. /Unive Thi-Qar

Abstract
The aim of this study is detecting antibodies of phase I and phase II of Coxiella burnetii bacterium, the cause of Q-fever, a zoonotic diesase in humans and animals in Thi-Qar province.Out of 393 serum samples collected randomly from Thi-Qar province from aborted and non aborted cows and ewes, the results appeared that 29 (7.37%) samples of cows and ewes were seropositive for C. burnetii distributed as 16 seropositive samples of 172 cows (9.3%) and 13 seropositive samples of 221 sheep (5.8%).the most positive cases associated with abortion cases with ratio (92.3%) in ewes and (75%) in cows.

The immune status of rabbits primed with chicken thymic protein

I. M.S .Shnawa

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 20-25

The immune status of rabbits primed with chicken thymic protein

I. M.S .Shnawa
Coll. of Scie./ Unive of Babylon

Abstract
Chicken thymus glands were collected from three weeks old chicken through standard manual dissection technique .The glands were minced ,mortared ,centrifuged at 5000 RPM for 15 min .and supernatant was saved .Thymic protein were separated by PEG 6%( M.w 6000) solution .This protein is designed as chicken thymic protein (CTP). CTP was used a test immunogen and sensitizer .As an immunogen it was used in concentration ,1.1g/land 0.55 g/l for priming of rabbits using multi site injection protocol with Freunds complete adjuvant .Passive haemagglutination ,NBT ,LIF ,E- rosette and skin test were made on CTP primed rabbits and controls.It was found that CTP could acts as humoral immunogen ,T cell suppressor and LIF cytokine inducer. Thus CTP may contain lapin immunoregulatory protein

A study the effect of supplementation vitamin C and dietary calcium on: I- Some productive performance in two different strains of broiler chicks under heat stress conditions

AL- Tepery; K.N.Tahir; A. L. D. AL- Khauzai; A.S.M

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 10-19

A study the effect of supplementation vitamin C and dietary calcium on: I- Some productive performance in two different strains of broiler chicks under heat stress conditions

A. L. D. AL- Khauzai K.N.Tahir A.S.M*. AL- Tepery
Coll. of Vet.Med./ Univ.of AL-Qadissiyah

Abstract
This study was undertaken to evaluate the effect of adding vitamin C at (0,150 mg/kg feed) and dietary calcium at (0.9,1.1%) on some productive performance of two different strains of broiler (Ross and Hubbard).A total of one hundred ninety two birds(96 chicks for each strain) one day old unsexed were used in this study. Each strain distributed randomly to four groups (24) chicks for each treatment, two replicates of (12)chicks for each.First group was fed on basal diet supplemented with (0.0 vitamin C+0.9% calcium).Second group was fed on the same basal diet supplemented with (0.0 vitamin C +1.1% calcium).Third group was fed on basal diet supplemented with (150 mg vitamin C/kg feed+0.9% calcium) and fourth group was fed on basal diet supplemented with (150mg vitamin C/kg feed +1.1% calcium).The results of this study showed that growth performance of chicks was significantly improved in Co-supplemented vitamin C and calcium (1.1%) and there was no significant interaction between vitamin C supplementation and calcium on dressing percentage and mortality rate.

Dermatitis in sheep in Mosul area

S.O. Youkhana

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 6-9

Dermatitis in sheep in Mosul area

S.O. Youkhana
Coll.of Vet. Med./Unive. of Mosul

Abstract
Dermatitis in (25) sheep showing different distribution of the lesions were classified pathologically to acute, subacute, and chronic dermatitis. Grossly, the lesions were characterized by alopecia, erythema, exudation, ulceration, scale and crust formation. Histopathological sections revealed degeneration, spongiosis, intercellular edema with diffuse leukocytic infiltration predominantly neutrophils, lymphocytes and eosinophils in the dermal layer of the skin. The lesions were more progressive in chronic dermatitis.

Laparoscopic intrauterine insemination of Awassi ewes superovulated with equine chorionic gonadotropin.

O. I. Azawi; M. K. M. A. Al-Mola

AL-Qadisiyah Journal of Veterinary Medicine Sciences, Volume 9, Issue 2, Pages 1-5

Laparoscopic intrauterine insemination of Awassi ewes superovulated with equine chorionic gonadotropin.

O. I. Azawi M. K. M. A. Al-Mola
Coll. of Vet. Med. Unive. of Mosul,
Abstract
The present study was undertaken to investigate the contribution of laparoscopic insemination to the improvement of fertilization and embryo recovery. The experiment was conducted in breeding season. Twelve nonpregnant and cycling Awassi ewes of 3-4 years of age were randomly allocated in equal number (n = 6) to two groups. Each ewe was treated with progesterone impregnated intravaginal sponge for 12 days. All ewes were superovulated with eCG (equine chorionic gonadotropin) 1200 IU once by intramuscular injection 48 h prior to sponge removal. Ewes of group 1 were mated naturally at least two times with Awassi rams of proven fertility. Ewes of group 2 had intrauterine insemination and were conducted 44-46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of semen in the distal portion of each uterine horn. Ovarian response was assessed by determining number of corpora lutea by laparoscopy on day 6 after mating. Embryo recovery was performed by semi-laparoscopic and by flushing of both uterine horns. Results of the present study revealed high number of unfertilized ova (P<0.05) was observed in ewes when naturally inseminated than ewes inseminated intrauterine using laparoscopic technique. Higher rate of embryo recovery (P<0.05) was achieved when ewes inseminated via intrauterine (4.66 ± 0.66) than ewes naturally mated (2.16 ± 0.74). The fertilization rate in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 79.93% and 40.07%, respectively. Fertilization failure in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 7.37% and 49.35%, respectively. It could be concluded that the use of eCG to induce superovulation in Awassi ewes combined with laparoscopic intrauterine insemination can increase the fertilization rate.