Study of gastrointestinal nematodes resistance to some anthelemitics and evaluation the efficacy of condensed tannin extraction on resistant genera in sheep in Babil
AL-Qadisiyah Journal of Veterinary Medicine Sciences,
2011, Volume 10, Issue 2, Pages 77-85
AbstractThe study was carried out in Babel province, where three sheep farms evaluated for albendazole, levamisol and ivermectin resistance in nematodes by in vivo and in vitro techniques as well as to demonstrate the efficacy of commercial tannin-rich solution on the resistant genera.According to low confidence interval limit of 95% fecal egg count reduction FECR ,In farm I, the animal which treated with levamisol and ivermectin considered as suspected resistant, while the animals which treated with albendazole showed resistance to it. In farm II ,the animal which treated with levamisol and albendazole were considered as suspected resistant ,while no FECR% <95 % was recorded in animals which treated with ivermectin ,and in the same manner ,the animals of farm III which treated with levamisol and albendazole showed suspected resistance and resistance respectively, and no resistance to ivermectin was recorded in animals which treated with it. Also, the results revealed that the combination between in vitro (EHA) and in vivo (FECRT) necessary to confirm the assessment of resistance against benzimedazoles. The most common genus identified from pre treatment feces were Haemonchus ,Trichostrongylus and Cooperia ,while Haemonchus was the only identified genus which demonstrated from post treatment fecal culture.The compared of in vitro effect of condensed tannin CT and thiabendazol revealed that both thiabendazole and CT induced significant egg hatching inhibition in a dose-dependent manner. The thiabendazole required a maximum of 0.5mg/ml, whereas, the CT required a maximum concentration of 2 mg/ml, to induce 100% egg hatch inhibition. The adult worm motility assay showed that the thiabendazole kill all worms at the concentration of 0.25 mg/ml, while CT was showed insignificant killing even in maximum concentration.
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