Molecular identification of Pasteurella multocida and their serotypes isolated from cattle and sheep in Diwanyia city
AL-Qadisiyah Journal of Veterinary Medicine Sciences,
2012, Volume 11, Issue 2, Pages 66-76
AbstractDue to the multi-similarities in phenotypic and biochemical characteristics among genera belong to pasteurellaceae , this study was aimed to isolate and diagnosis of (Pasteurella multocida) that cause respiratory infection in cattle and sheep by using routine methods (culture and biochemical) , then used of molecular method as a diagnostic confirmatory, in addition to conduct the serotyping by using polymerase chain reaction , The study included acollection of (150) samples of infected lungs and smears of nasal , tonsils swabs of cattle and sheep for the period 1-11-2010 and up to 1- 4-2011 of farm animals and various massacres in the city of Diwaniya.Samples were cultured on the blood agar and MacConkey agar and Trypticase Soya agar then diagnosed after pure isolation of colonies using phenotypic and biochemical methods.The results of polymerase chain reaction (PCR) as confirmatory test isolates after extraction of DNA from isolates and amplification of specific known as KMT-1the presence of asingle band for the amount of amplified DNA with amolecular weight of 460bp.For the purpose serotyping of isolates germ of Pasteurella multocida using PCR, the capsule specific primer (CAPA, CAPB, CAPD, CAPE, CAPF)were used showed that the serotype (B) was the dominat in cattle , with molecular weight (760pb) while type (A) the dominat in sheep with molecular weight (1044pb).The Coclusion , the result of molecular level of identification and serotyping gave ahigh sensitivity (97) % and specificity (82.05) % when compared with its routine diagnostic in cattle and sheep.
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