Rapid detection of infectious bronchitis virus in broilers in Al-Diwaniya governorate by using Real-Time reverse transcriptase Polymerase Chain Reaction
AL-Qadisiyah Journal of Veterinary Medicine Sciences,
2015, Volume 14, Issue 1, Pages 22-26
AbstractInfectious Bronchitis (IB) is currently one of the most important viral diseases in poultry flocks all over the world caused by infectious bronchitis virus (IBV), and it causes huge economic losses in poultry industry. Infectious bronchitis virus has many serotypes that do not confer cross protection against each other. This study was conducted to detect Infectious bronchitis virus in broilers chicken farms in Diwaniya governorate. Tracheal swab from 30 infected chicken flocks located in different areas of Diwaniya governorate were collected to make Rapid immunochromatography test for IBV by using Anigen Rapid IBV Antigen Test Kit and tissue samples were collected from flocks which showed positive for rapid test to make rRT-PCR. Twenty eight (93.33%) flocks were positive for IBV by rapid immunochromatography test. The molecular detection of IBV showed that all flocks (100%) were positive for IBV. In conclusion the IBV was the most important cause of respiratory diseases in this study. The real-Time RT-PCR was found very efficient and rapid in detection of IBV in infected chickens. .
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