AL-Qadisiyah Journal of Veterinary Medicine Sciences

Abstract

Study the activity of the bull's frozen semen thawed in certain diluents

Khalid M.Karam-College of Veterinary Medicine-University of Al-Qadisyah

Abstract

This study was represented by taking 120 micro straws (0.25 ml) filled of frozen semen produced in the Artificial insemination center /main center –Abu Ghraib ,which belongs to the General Public Establishment for the Animal Resources service ,Ministry of Agriculture ,Iraq ,which is diluted primarily by Tris and Glycerol 1:15.
These specimens had been thawed at 0 ºC ,20 ºC , 37 ºC after redilution the following diluents at ratio 1: 6 and storing till 6 hours.
1-control group (without diluents ).
2-Soduim citrate solution 2.8% .
3-Soduim chloride solution 0.9% .
4-Lactose solution 11% .
The specimen were examined every 3 hours starting from zero time by evaluation of spermatozoal motility percentage and the spermatozoal live and dead account percentage.
The results showed that rediluting with sodium citrate 2.8% solution and lactose 11% solution gave best results then control group and redilution with sodium chloride 0.9% solution in both spermatozoal motility percentage and spermatozoal live and dead percentage ,and thawing at 37 ºC gave best results compared with other thawing temperatures (0 ºC ,20 ºC) and this study showed that when ever storage time is increases will reduce spermatozoal activity.