Immunopathological evaluation of the immunity induced by whole bacteria sonicated antigen ofBrucella melitensis in guinea pigs
AL-Qadisiyah Journal of Veterinary Medicine Sciences,
2009, Volume 8, Issue 2, Pages 42-55
AbstractImmunopathological evaluation of the immunity induced by whole bacteria sonicated antigen of
Brucella melitensis in guinea pigs
Al-Oubaidy, S.S.(1); Alwan, M.J.(2); Al-Kaisei, B.I.(1)
(1) Department of pathology and poultry, (2) Zoonosis unit / Collage of Veterinary Medicine / University of Baghdad.
In order to know the efficacy of whole Bacterial Sonicated Antigens (WBSAgs) of Brucella on immune response in guinea pigs. Eighteen (18) guinea pigs ( both sex ) were divided randomly to three groups: 1st group (n=6) was immunized with whole bacterial sonicted antigens (WBSAgs) of Brucella melitensis (0.5ml/ 1mg protein) S/C and repeated after 15 days in the same dose, the 2nd group (n=6) and the 3rd group (n=6) served as positive and negative control respectively.
Cell mediated and humoral immune responses were checked at day 27 and 30 post immunization respectively. After 30 days post immunization, the 1st, and 2nd groups were challenged I/P with 1ml containing 2x109 CFU/ml of viable virulent B. melitensis and 3rd group inculated with 1ml sterile normal saline.
Three animals from each groups were sacrificed on day 10 and 30 post-challenge.
The result showed that immunization with the WBSAgs of B. melitensis result in production of cellular and humoral immune response, as examined by delayed type hypersensitivity (DTH) and indirect heamagglutination test.
Abortion was found in the non-immunized infected animals, where as in the immunized infected animals was not reported.
Mild to moderate bacterial isolation and pathological changes were recorded in the target organs of the immunized infected animals at 10 days post infection, negative or very mild bacterial isolation with few splenic bacterial counts were obtained from the target organs of 1st with hyperplastic lymphoid tissue in their organs at 30 days post infection.
The control positive group showed very heavy bacterial isolation with intense sub-acute to chronic inflammatory reaction were noticed in the examined organs at 10 days post infection, and heavy bacterial isolation with high bacterial counts of spleen were investigated at 30 days post infection.
Mature to epithelioid granulomatous lesions were the main lesion in the target organs of non-immunized infected animals at 30 days post infection.
We concluded that the WBSAgs of B. melitensis induce a good protective immune response against the challenge with B. melitensis.
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