Print ISSN: 1818-5746

Online ISSN: 2313-4429

Keywords : time PCR

Detection of shiga toxin producing Escherichia coli isolated from mastitis milk of sheep by qPCR technique

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2016, Volume 15, Issue 2, Pages 1-5

This study was conducted to determine the Shiga toxin producing Escherichia coli isolated from mastitis milk samples of sheep that collected from Diwanyia city by qPCR technique. A total of (60) clinical investigated mastitis milk samples from sheep were subjected to bacterial DNA extraction by using (PrestoTM Mini gDNA Bacteria Kit). The extracted DNA subjected to Real-Time PCR technique (qPCR) for detection of shiga toxin 1 (stx1) gene. Results were revealed that the mastitis sheep are more prevalence for shedding shiga toxin 1 producing Escherichia coli (13.3%) of (8/60) positive samples. In conclusion, the Real-Time PCR technique was shown high specific and rapid method in detection of shiga toxin gene and the sheep which infected by mastitis can be carried the shiga toxin producing Escherichia coli, that may be considered to be risk factor of human infections..

Identification of Campylobacter jejuni in poultry products by Real-Time PCR in Al-Muthanna province

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2015, Volume 14, Issue 2, Pages 1-6

The study was undertaken due to the potential contribution of poultry products is less well known in Iraq. In this study, Campylobacter jejuni identified by application SYBR green based Real-Time PCR technique by amplification the specific hipo gene in (96) chicken tissue samples. The results show no significant difference in detection rates between fresh (77.8%) and frozen (83.3%) poultry tissue samples. In freshly slaughtered poultry samples, the liver detection rate (93.75 %) was higher than skin (62.5%) and meat (75%) while in frozen poultry samples, the detection rate was higher in meat (93.75 %) than skin (87.5%) and liver (68.75%). In conclusion the study showed high contamination rates of poultry with Campylobacter jejuni in retail markets and the direct Real-Time PCR is suitable assay for screening poultry products and identification of Campylobacter jejuni pathogen. Campylobacter jejuni.

Rapid detection of Yersinia enterocolitica by using Real-Time PCR technique in some types of foods in Al-Qadisiya province

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2015, Volume 14, Issue 1, Pages 34-38

The present study was carried out for direct and rapid detection of pathogenic Y. enterocolitica in some types of foods such as (raw milk, local sweet cheese and minced beef meat) exist in local different markets of Al-Qadisiya province. A total of 192 samples were collected randomly from markets of Al-Qadisiya province involved 79 raw milk samples, 58 local sweet cheese samples and 55 minced beef meat samples. Real-Time PCR technique based SYBER Green dye were used for detection of this bacteria by amplifying invasion locus protein (ail) gene which is found especially in Y. enterocolitica, The results revealed that positive samples for this (ail) gene were 49 samples out of 79 examined raw milk (62%), also 6 samples out of 58 samples observed in local sweet cheese (10.3%) and 49 samples out of 55 samples of minced beef meat (89%). A total of positive samples were 104 out of 192 examined samples, samples of minced beef meat recorded highly significant contamination (p<0.05) as compared samples of raw milk which occupied second rank in contamination with pathogen and then samples of local sweet cheese which recorded less degree of contamination. The Real-time PCR technique was very specific and efficient for detection Y. enterocolitica in variety foods, when compared with culture method which have many problems..