Print ISSN: 1818-5746

Online ISSN: 2313-4429

Keywords : raw milk


Rapid detection of Yersinia enterocolitica by using Real-Time PCR technique in some types of foods in Al-Qadisiya province

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2015, Volume 14, Issue 1, Pages 34-38

The present study was carried out for direct and rapid detection of pathogenic Y. enterocolitica in some types of foods such as (raw milk, local sweet cheese and minced beef meat) exist in local different markets of Al-Qadisiya province. A total of 192 samples were collected randomly from markets of Al-Qadisiya province involved 79 raw milk samples, 58 local sweet cheese samples and 55 minced beef meat samples. Real-Time PCR technique based SYBER Green dye were used for detection of this bacteria by amplifying invasion locus protein (ail) gene which is found especially in Y. enterocolitica, The results revealed that positive samples for this (ail) gene were 49 samples out of 79 examined raw milk (62%), also 6 samples out of 58 samples observed in local sweet cheese (10.3%) and 49 samples out of 55 samples of minced beef meat (89%). A total of positive samples were 104 out of 192 examined samples, samples of minced beef meat recorded highly significant contamination (p<0.05) as compared samples of raw milk which occupied second rank in contamination with pathogen and then samples of local sweet cheese which recorded less degree of contamination. The Real-time PCR technique was very specific and efficient for detection Y. enterocolitica in variety foods, when compared with culture method which have many problems..

Determination the levels of cadmium and lead in raw milk of cows, sheep and goats in Al-Qadisiya province

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2014, Volume 13, Issue 2, Pages 16-22

This study was undertaken in Al-Qadisiya province during the period from December 2012 to March 2013 to determine the levels of cadmium and lead in raw milk of cows, sheep and goats in compared with the permissible levels reported by the international organizations. The study was included the analysis of (150) raw milk samples (50) samples per each species of animals from different regions of the province involving Diwaniya center, Sanyia, Shafeia Daghara, and Al-Hamza, (10) samples from each region. Results of the statistical analysis showed that there was a significant differences ( p˂0.05) in levels of Cadmium (Cd) and Lead (Pb) among the studied animals, the highest level of Cadmium and Lead were (0.251 and 0.801) mg /L respectively for sheep milk and the lowest values (0.098 and 0.311) mg / L respectively for cow's milk, and also shown significant difference (P<0.05) among different regions where the highest level of Cadmium and Lead were (0.184 and 0.624) mg / L respectively for AL-Hamza district and the lowest level were (0.144 and 0.507) mg / L respectively for AL-Daghara region. These values were a higher than permissible levels (0.005) ppm for Cadmium and (0.02) mg / L of milk for Lead .