Print ISSN: 1818-5746

Online ISSN: 2313-4429

Keywords : Diarrhea

Detection of hemolysin virulence factor gene of Enterohemorrhagic Escherichia coli (EHEC) isolated from feces of infected sheep by using Polymerase Chain Reaction Technique

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2015, Volume 14, Issue 1, Pages 127-131

Enterohemorrhagic Escherichia coli (EHEC) are the most important recently emerged group of food-borne pathogens. It can cause severe gastrointestinal disease, including fatal infections, and is being detected more frequently worldwide. In this study the Polymerase chain reaction technique (PCR) were used for detection of hemolysin toxin gene (hlyA) as virulence factor producing by Enterohemorrhagic Escherichia coli (EHEC) isolated from diarrheic sheep. The PCR primers for hlyA gene were designed according to a program from NCBI-Genbank published sequence (Genbank code: X94129.1). Fifty fecal samples were collected from sheep suffering from diarrhea, (32) isolates (64%) of Escherichia coli were detected and diagnosed by routine laboratory tests. The results were shown only (13) positive isolates in PCR technique for detection of hemolysin toxin gene at (40.62%) which present as Enterohemorrhagic Escherichia coli out of (32) E. coli. In conclusion, hemolysin toxin gene (hlyA) is important virulence factor of Enterohemorrhagic Escherichia coli infected sheep and using PCR was appeared very sensitive and highly specific assay serve as suitable molecular diagnostic tool for detection Enterohemorrhagic Escherichia coli producing hemolysin toxin..

Detection of bovine rotavirus in diarrheic calves by using rapid test in some Mid-Euphrates provinces

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2014, Volume 13, Issue 2, Pages 20-26

The study was aimed to determine the Rota viral infection rates in diarrheic calves and effectiveness of some epidemiological factors on that rate in some provinces in Mid–Euphrates region in Iraq (Babylon, Al-Najaf, Karbala, Al-Qadisyia provinces). A total of 530 calves aged between 1-60 day were examined and fifty fecal (50) samples were collected from diarrheic calves only within six months period from November-2012 to April -2013. The fecal samples were examined for the presence of rotavirus by rapid rotavirus test (screening test), as well as the clinical examination for these diarrheic calves were done. The rapid test demonstrated that the rotavirus infection rate was 18/50 (36%). A 45.4% of rotavirus infection was found in calves less than 30 days of age which was significantly higher than 28.5% calves (30-60) days old. Male and female (37.5%, 40% respectively) were seen infected without any significant differences. The main clinical signs observed on rotavirus diarrheic calves were include, watery voluminous diarrhea with profound weakness and mild depression, so as temperature, respiration, heart rates could be decreased and appetite was changeable towards loss or anorectic. In Babylon, the highest rotavirus infection rate was recorded 60%, and less rates in Karbala 20% and 40%, 33% in AL-Najaf and AL-Qadisyia provinces respectively. The rotavirus infection rate in diarrheic calves was affected by months significantly, which it was higher at January 75%, so as in November 50%, December 40% and February 33.3%, but not recorded in March and April..

Effect of enterocin produced by Enterococcus faecalis on bacteria that cause diarrhea in children and young sheep

H. S. Awayid; Kh.M.Khamas

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2013, Volume 12, Issue 1, Pages 32-44

A total of 300 samples (100 samples from feases healthy adult persons, adult persons suffer from diarrhea and100 feases healthy child and from feases children suffer from diarrhea and 100 samples from feases young sheep suffer from diarrhea ) , taken from various hospitals in the Baghdad city during the period from January to April 2012. All isolates were subjected to the cultural, microscopical, biochemical examinations by Vitek 2 for identification up to the species. The results showed that 40 isolates belonged to Enterococcus faecalis of which, 25 isolates from diarrhea cause and 15 isolates from normal flora , 31 isolates of which 18 isolates belonged to E.coli and 5 isolates belonged to E.coliO157:H7 and 8 isolates belonged to Salmonella spp. All isolates were subjected to cultural ,microscopical, biochemical examinations and used( API 20 –E) strip. and sensitivity of 31 isolates was tested against (11-9) Antibiotics. Results revealed that isolates showed multi resistance to antibiotics, All isolates of E.coli and E.coliO157:H7 ,Salmonella spp.resistant to some antibiotics for negative Bacteria and belonging to the group Enterobacteriaceae. And Detection of the ability of E. faecalis local isolates to produce enterocin by testing the inhibitory activity agar and broth in two media MRS,BHI and two method against bacteria cause diarrhea. And The results showed a variety of the local isolates in their inhibitory effect against bacteria cause diarrhea in children and young sheep by inhibitor zone between (12-20) mm and was MRS liquid and solid the best media from the brain heart infusion .

Detection of Campylobacter spp. in children diarrhea by using Polymerase Chain Reaction PCR technique in Al-Diwanyiah Governorate.

A. H. AL-Hamadani; Z.F.Saleh

AL-Qadisiyah Journal of Veterinary Medicine Sciences, 2011, Volume 10, Issue 2, Pages 45-54

This study was conducted in order to identify of Campylobacter spp. As a causative agent of diarrhea in children using routine laboratory diagnosis (direct and culture methods) in comparison with polymerase chain reaction (PCR) technique as a confirm diagnostic tool.A total of 100 children stool samples were collected from both sexes at ages less than two years olds suffering from diarrhea who admitted the maternity and Pediatric Teaching hospital in Al-Diwaniyiah Governorate from December 2007 to August 2008.Based on the clinical and laboratory diagnosis, results revealed that the percent of Campylobacter isolation was 8% included C. coli and C. jejuni for children samples. In addition, the results haven't revealed any statistically significant (P≥0.01) between the rate of infection and sexes, while there was a statistically significant (P≤0.01) between these rates and ages, where it noted that patients (>1) years old were more prone to infect with Campylobacter spp. exposure to infections.The results revealed that the PCR positive samples contained one band of amplified DNA with molecular weight (816 bp) after electrophoresis and examined under UV- transilluminator. The study also showed that the sensitivity and specificity of PCR technique were 40% and 100% respectively for examination children samples, when compared with direct examination, but were with culture method were 33% and 100%; respectively in children.